Study of scFv-therapy that mimicking the gp43 antigen from Paracoccidioides brasiliensis combined with nanoparticle in experimental paracoccidioidomycosis

Abstract

The paracoccidioidomycosis is a deep mycosis, caused by Paracoccidioides brasiliensis (Pb) that attack the lung primarily. This fungus produce a several molecules and the gp43 is the main antigenic component of the Pb. Because the importance of the gp43, monoclonal antibodies were done. Based in the idiotypic network hypothesis, Jerne (1974) suggested that the antibody produced in response to some antigen, induce the others antibody production against the variable chain. Antibodies against idiotopes that recognize the paratope, are known Ab2- beta. This fragment has been used in several therapeutic models (PAN, et al., 1995, BONA, 1996 e HERLYN et al., 1996). In spite of the antibody to be a complex molecule, we know that the Fab region is the responsible to mimmick the antigen. Our group (FERREIRA, et al., 2011) engineered a new molecule, that mimmicking the gp43 antigen of Paracoccidioides brasiliensis, the single chain fragment variable (scFv). The transfection of the scFv in dendritic cells was competent in the experimental PCM treatment. The scFv trasfected in dendritic cells was capable to induce T cells response and reduced the unit forming colonies in the lung. On the order hand, the nanoparticle can stimulate for a long time the scFv in the animal. So, the aim of this work is to insert the scFv in the nanoparticle to high the efficiency of the therapy in experimental PCM. (AU)