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Evaluation of DNA damage in Anesthesiologists

Grant number: 13/05084-8
Support Opportunities:Regular Research Grants
Duration: July 01, 2013 - December 31, 2015
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Leandro Gobbo Braz
Grantee:Leandro Gobbo Braz
Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated researchers:Mariana Gobbo Braz


The human monitoring has been used as a useful tool to identify and quantify the risk related to harmful effects in health. There is a few data in Brazil regarding the monitoring of occupational exposure to waste anesthetic gases, without national data of waste anesthetic gases determined in operating room (OR). This proposal aims to determine the concentrations of waste anesthetic gases in the OR and evaluate DNA damage in exposed medical personnel. The study will be performed in the OR of the Hospital of Botucatu Medical School - UNESP and in 60 subjects: 30 anesthesiologists exposure to waste anesthetic gases and 30 physicians without occupational exposure, matched by age, gender and lifestyle. The concentrations of isoflurane, sevoflurane, desflurane and nitrous oxide (waste anesthetic gases) will be determined in the OR using a portable infrared spectrophotometer. Venous blood samples and buccal cells will be collected from all professionals. DNA damage evaluated in lymphocytes will be detected by comet assay and chromosomal damage by buccal micronucleus cytome assay. Taken together, data of the values of waste anesthetic gases in OR and their possible effects on genetic material will be assessed in anesthesiologists. This knowledge will certainly contribute to future preventive actions, adequating the concentrations of waste anesthetic gases to international standards and reducing the possible damage into the exposed professionals, with direct impact on public health. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRAZ, MARIANA G.; SOUZA, KATINA M.; LUCIO, LORENA M. C.; DI RENZO, GIULIA C. C.; FELICIANO, LUCIANA M.; MARCONDES, JOAO PAULO C.; OLIVER CHEN, C. -Y.; BRAZ, JOSE REINALDO C.; BRAZ, LEANDRO G.. Detrimental effects detected in exfoliated buccal cells from anesthesiology medical residents occupationally exposed to inhalation anesthetics: An observational study. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 832, p. 61-64, . (13/21130-0, 13/05084-8)
SOUZA, KATINA M.; BRAZ, LEANDRO G.; NOGUEIRA, FLAVIA R.; SOUZA, MARAJANE B.; BINCOLETO, LAHIS F.; AUN, ALINE G.; CORRENTE, JOSE E.; CARVALHO, LIDIA R.; BRAZ, JOSE REINALDO C.; BRAZ, MARIANA G.. Occupational exposure to anesthetics leads to genomic instability, cytotoxicity and proliferative changes. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, v. 791, p. 42-48, . (13/05084-8)
LEANDRO GOBBO BRAZ; JOSÉ REINALDO CERQUEIRA BRAZ; GUILHERME APARECIDO SILVA CAVALCANTE; KÁTINA MENEGHETTI SOUZA; LORENA MENDES DE CARVALHO LUCIO; MARIANA GOBBO BRAZ. Comparação de resíduos de gases anestésicos em salas de operação com ou sem sistema de exaustão em hospital universitário brasileiro. Revista Brasileira de Anestesiologia, v. 67, n. 5, p. 516-520, . (13/05084-8, 13/21130-0)
BRAZ, MARIANA G.; SILVA, MARIANE A. P.; FIGUEIREDO, DRIELLE B. S.; AUN, ALINE G.; MARQUES, LAIS S. K.; LARA, JULIANA R.; BRAZ, LEANDRO G.. Genetic instability assessed by telomere length and micronucleus in physicians exposed to anesthetics. Environmental and Molecular Mutagenesis, . (13/21130-0, 13/05084-8, 16/15559-1)

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