This project is a collaborative research within the BIOEN -FAPES P/FAPEMIG call between the Biotechnology Department of the Lorena School of Engineering (EEL/USP), Physics Department of the Federal University of Juiz de Fora (UFJF), Microbiology Department of the Federal University of Minas Gerais (UFMG) and Microbiology Laboratory, São Paulo State University (UNESP Rio Claro). The project aims at the fractioning of sugarcane bagasse in its main components (cellulose, hemicelluloses and lignin) for their use in the production of ethanol. The acid hydrolysis will be used to remove the hemicellulosic fraction followed by alkaline hydrolysis. The enzymatic hydrolysis of the cellulose fraction will be performed. The hydrolysates will be characterized by advanced spectroscopic techniques as Raman scattering, infra red absorption with Fourier transformation (FTIR), absorption in the near infra-red ray (NIR), thermal lens and photo-acoustic. The use of these techniques is interesting due to their nondestructive character, possibility of in situ measurements and further development of compact prototypes. In the further stage, the xylose and glucose rich hydrolysates will be properly treated by detoxification methods and used as fermentation media for the production of ethanol by xylose-fermenting yeasts and S. cerevisiae, respectively. Xylose-arabinose fermenting species isolated from the Atlantic Rain Forest, Amazon Forest and Brazilian Cerrado ecosystems will be tested. Yeasts inhabiting rotting-wood substrates will be collected and tested for xylose-arabinose fermentation. This project aims at finding new species from these Brazilian ecosystems capable to be used in industrial processes. In all the involved unitary operations in this study, conditions will be optimized by experimental design and data analysis by means of appropriate statistical methodologies. Obtained results will allow establishing advanced technologies and innovations in order to extend the national and international competitiveness of “bioenergy” to reach second generation technologies for alcohol program. It will allow the formation of teams and cooperation among the participant institutions for training and exchange of knowledge. (AU)
Articles published in Agência FAPESP Newsletter about the research grant:
GIESE, ELLEN C.;
DUSSAN, KELLY J.;
CHANDEL, ANUJ K.;
PAGNOCCA, FERNANDO C.;
DA SILVA, SILVIO S.
Cellulase Production by Trichosporon laibachii.
ORBITAL-THE ELECTRONIC JOURNAL OF CHEMISTRY,
Web of Science Citations: 1.