Use of ultrasound to evaluate mesenchymal stem cells transplant, in the equine muscle, quantum dots labeled and submitted to the lasertherapy

Abstract

Researches with mesenchymal stem cells (MSCs) and tissue engineering have shown that prospects will likely benefit the health of both humans and domestic animals, among them performance horses. The high differentiation potential of MSCs in different cell types, it's easy expansion in culture and the possibility of transplants have led to increased research into these cells. The therapeutic interest in MSCs is related to their migratory potential, the paracrine effects, the anti-inflammatory and immunomodulatory as well as in their multipotentiality. However, in horses, many of its biological characteristics remain unknown, among them the residence time at the site of transplantation. Several studies have been conducted to better understand the biology of MSCs, however, researches in the equine species are scarce. In this context the use of cell markers is essential for tracking these cells. There are many cellular markers described for this purpose, Fluorescent Lipophilic Chloromethylbenzamido (CM-DiI), Green Fluorescent Protein (GFP), Quantum dots (QDs), Micron-Sized Iron Oxide Particles (MPIOs), among others. This study aims to compare images obtained through ultrasound associated with laser therapy of MSCs marked and unmarked with Nanocrystal Qtracker 655 ®, respectively inoculated into muscle brachiocephalicus left and right of 10 adult horses, with the aim of assessing the potential traceability of the method employed. (AU)