Ionizing radiation and resveratrol effects in lung cancer cells culture

Abstract

Mucoepidermoid carcinoma of the lung is a histological type that derives from tracheobronchial mucous glands. It is manifested with obstructive symptoms and tends to be locally invasive compromising the trachea. Curative or palliative purpose for the disease, nowadays presents a strong tendency to develop therapeutic strategies aimed at the administration of compounds with high potential for optimizing the ionizing radiation effect from the radiation therapy, in order to enhance the killing of tumor cells and preserve intact cells of adjacent normal tissues. The intensive search of such strategies showed quite promising results presented by the compound called Resveratrol, making it widely available and subject of intense studies. Resveratrol (3,4',5-trihydroxystilbene) is a polyphenol belonging to the compounds group called phytoalexins. It shows a high antioxidant potential provided by its ability to capture the free radicals presented in the intracellular environment. The main objective of this study is to determine in vitro the resveratrol effect in cell culture of mucoepidermoid carcinoma of the lung exposed to different doses and dose rates of ionizing radiation from Linear Accelerator (LINAC). For this purpose it will be performed in cell culture line NCI-H292 from ATCC both tests: the resveratrol toxicity level found by cytotoxicity test performed by neutral red uptake assay, as well as the lethal dose 50% (LD50) of ionizing radiation. Based on the IC50% and the LD50 results, tests will be performed under the same experimental conditions. They will be performed using the immunofluorescence assay in order to evaluate the induction of the cellular apoptosis and necrosis, and the presence of the DNA double break repair after the cells were exposed to different doses and dose rates of ionizing radiation, and also in the presence and absence of resveratrol. (AU)