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Identification, by microarray, of transcriptional predictors of the gains in cardiorespiratory functional capacity

Abstract

The extent of the benefit caused by non-pharmacological interventions, as in with exercise training, varies substantially among individuals. The adaptations of the different physiological systems to the stressor stimulus of physical exertion, partially measurable by the cardiorespiratory functional capacity (CRFC) analysis, in fact reflect the synchronic action among genes, scenery that may be explored from global gene expression analysis by the microarray method. This study proposes to identify new genes modulators of the CRFC phenotype and to identify a differential gene expression pattern among individuals, in leukocytes and skeletal muscle tissue. Also, to investigate the possible existent correlation between these "molecular signatures" and the gains of CRFC in both tissue compartments. Our hypothesis is that the nuclear reprogramming in leukocytes induced during physical exertion is well characterized as a fine systemic biological sensor of those alterations occurring at skeletal muscle tissue (local biological sensor), excluding the demand for muscle biopsy. Until the present moment, the similarities, the differences and how representative are the "training responsive transcriptomes" of these two tissues are not known, knowledge that will potentially serve as a basis for individualized therapies. To test our hypotheses, the work will be conducted using 40 healthy sedentary recruits of the São Paulo Estate Military Police, from a partnership settled in about 6 years for research purposes. They will be submitted to an exercise training protocol for 18 weeks and will be evaluated before and after this intervention period by: CRFC evaluation by ergospirometry, blood sampling and skeletal muscle biopsy for transcriptome analysis. (AU)

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