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Immunoproteomics applied to the diagnosis human strongyloidiasis used antigen Strongyloides venezuelensis

Grant number: 13/04236-9
Support Opportunities:Regular Research Grants
Duration: May 01, 2013 - August 31, 2015
Field of knowledge:Biological Sciences - Parasitology - Helminthology of Parasites
Principal Investigator:Ronaldo Cesar Borges Cryschek
Grantee:Ronaldo Cesar Borges Cryschek
Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers:Fabiana Martins de Paula ; Marcelo Andreetta Corral ; Pedro Paulo Chieffi

Abstract

Strongyloidiasis is a potentially serious infection in immunocompromised patients. Parasitological diagnosis by traditional techniques has low sensitivity. Thus, the availability of sensitive and specific diagnostic techniques is desirable, especially in the context of immunossupression, where the identification and treatment of strongyloidiasis is mandatory. Therefore, it is proposed to verify the application of immunoenzymatic (ELISA) serological techniques and Western blotting (WB) using soluble fractions of the crude extract of evolutionary stages, filariform larvae and adult female worm of Strongyloides venezuelensis for the diagnosis of human strongyloidiasis. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CORRAL, MARCELO ANDREETTA; DE PAULA, FABIANA MARTINS; MEISEL, DIRCE MARY C. L.; ABDALA, EDSON; COSTA, SILVIA FIGUEIREDO; PIERROTTI, LIGIA CAMERA; YAMASHIRO, JULIANA; DO NASCIMENTO GONCALVES, ELENICE M.; CASTILHO, VERA LUCIA P.; CHIEFFI, PEDRO PAULO; et al. IgG reactivity with 40-35 kDa soluble and membrane antigen of Strongyloides venezuelensis in immunocompromised patients. Acta Tropica, v. 190, p. 357-360, . (10/51110-2, 13/04236-9)
CORRAL, M. A.; PAULA, F. M.; MEISEL, D. M. C. L.; CASTILHO, V. L. P.; GONCALVES, E. M. N.; LEVY, D.; BYDLOWSKI, S. P.; CHIEFFI, P. P.; CASTRO-BORGES, W.; GRYSCHEK, R. C. B.. Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens. Parasitology, v. 144, n. 2, p. 124-130, . (13/04236-9)
TOLEDO, BEATRIZ; CORRAL, MARCELO A.; MEISEL, DIRCE MARY C. L.; GOTTARDI, MAIARA; ABDALA, EDSON; COSTA, SILVIA F.; PIERROTTI, LIGIA CAMERA; LESCANO, SUSANA A. Z.; GONCALVES, ELENICE M. N.; CASTILHO, VERA L. P.; et al. Screening of Strongyloides infection using an ELISA test in transplant candidates. Clinics, v. 74, . (13/04236-9, 10/51110-2)
ROLDAN GONZALES, WILLIAM HENRY; COELHO, GUILHERME RABELO; PIMENTA, DANIEL CARVALHO; DE PAULA, FABIANA MARTINS; BORGES GRYSCHEK, RONALDO CESAR. Proteomic analysis of the excretory-secretory products from Strongyloides venezuelensis infective larvae: new insights for the immunodiagnosis of human strongyloidiasis. Parasitology Research, v. 121, n. 11, p. 16-pg., . (13/04236-9)
CORRAL, MARCELO ANDREETTA; PAULA, FABIANA MARTINS; GOTTARDI, MAIARA; CORREIA LIMA MEISEL, DIRCE MARY; CHIEFFI, PEDRO PAULO; BORGES GRYSCHEK, RONALDO CESAR. Frações de membrana de Strongyloides venezuelensis para o imunodiagnóstico da estrongiloidíase humana. Revista do Instituto de Medicina Tropical de São Paulo, v. 57, n. 1, p. 77-80, . (13/04236-9)
GRYSCHEK, RONALDO CESAR BORGES; CORRAL, MARCELO ANDREETTA; SITTA, RENATA BARNABE; GOTTARDI, MAIARA; PIERROTTI, LIGIA CAMERA; COSTA, SILVIA FIGUEIREDO; ABDALA, EDSON; CHIEFFI, PEDRO PAULO; DE PAULA, FABIANA MARTINS. Strongyloides infection screening in transplant candidates: What is the best strategy?. TRANSPLANT INFECTIOUS DISEASE, v. N/A, p. 8-pg., . (10/51110-2, 13/04236-9)
CORRAL, MARCELO ANDREETTA; DE PAULA, FABIANA MARTINS; GOTTARDI, MAIARA; CORREIA LIMA MEISEL, DIRCE MARY; PAGLIUSI CASTILHO, VERA LUCIA; DO NASCIMENTO GONCALVES, ELENICE MESSIAS; CHIEFFI, PEDRO PAULO; BORGES GRYSCHEK, RONALDO CESAR. Imunodiagnóstico da estrongiloidíase humana: o uso de seis diferentes frações antigênicas de fêmeas parasitas de Strongyloides venezuelensis. Revista do Instituto de Medicina Tropical de São Paulo, v. 57, n. 5, p. 427-430, . (13/04236-9)
DE PAULA, FABIANA MARTINS; MALTA, FERNANDA MELLO; CORRAL, MARCELO ANDREETTA; MARQUES, PRISCILLA DUARTE; GOTTARDI, MAIARA; CORREIA LIMA MEISEL, DIRCE MARY; YAMASHIRO, JULIANA; REBELLO PINHO, JOAO RENATO; PAGLIUSI CASTILHO, VERA LUCIA; DO NASCIMENTO GONCALVES, ELENICE MESSIAS; et al. DIAGNOSIS OF Strongyloides stercoralis INFECTION IN IMMUNOCOMPROMISED PATIENTS BY SEROLOGICAL AND MOLECULAR METHODS. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, . (10/51110-2, 13/04236-9)
ROLDAN GONZALES, WILLIAM HENRY; MEISEL, DIRCE MARY CORREIA LIMA; DE PAULA, FABIANA MARTINS; GRYSCHEK, RONALDO CESAR BORGES. Diagnostic accuracy of somatic and excretory-secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis. Parasitology, v. 148, n. 12, p. 1522-1527, . (13/04236-9)

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