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Analysis of concentration and activity of carbonic anhydrase VI (Ca VI) in saliva and plaque in deciduous teeth and its relationship to dental caries: longitudinal study in children 3-6 years and transverse in children 7-9 years

Abstract

Previous studies has indicated that the buffering capacity of saliva (BCS), pH and salivary flow (SF) appear to be inversely related to caries prevalence. Additionally, previous studies have shown a negative correlation between the concentration of isoenzina carbonic anhydrase VI (CA VI) in saliva and caries prevalence in children and adults. However, the fact that AC VI is concentrated in a certain way, does not mean that every isoenzyme is biologically active to exert its effect. In addition, there are no reports in the literature of studies that have investigated the relationship between the activity of this isoenzyme, pH, salivary flow and buffering capacity and caries prevalence, as well as the activity of CA VI can be used as a predictor of caries risk in preschool children and school. Objectives: 1 - A) Determine the SF, the BCS and the CA VI isoenzyme activity in the saliva of preschool children with caries and caries-free before and after rinsing with a solution of 20% sucrose; B) Investigate the relationship between these variables and dental caries, and C) Investigating the power of these variables as predictors of risk of dental caries in preschool children after 01 year. 2 - A) Determine the concentration and activity of isoenzyme CA VI in saliva and dental plaque and salivary flow, pH and buffering capacity of saliva and dental plaque of school children with and without caries, and B) To investigate the relationship between these variables and dental caries. Materials and Methods: 380 preschool children aged 36-72 months of both genders will be selected in public daycare centers in Piracicaba, São Paulo, Brazil, which will be divided into two groups: caries-free group (LC group, n = 190) and caries group (Group C, n = 190) which will rinse with sucrose solution at 20% before and 5 minutes after the collection of samples and 100 students from 7 to 9 years will be divided into 02 groups: free caries (Group LC n = 50) and caries group (Group C, n = 50). Preschool children will be subjected to clinical examination by a calibrated examiner. In each individual will be checked for the presence of clinically visible biofilm on the upper incisors, the rate of decay, the activity of salivary CA VI, the SF and BCS before and 5 minutes after rinsing with a solution of 20% sucrose. In the school will also be performed clinical examination, collection of saliva and plaque for analysis of salivary flow and the variable concentration and activity of AC VI, analysis of pH and buffering capacity. For testing the concentration of salivary CA VI, we use procedures for labeling and fluoroimmunoassay antigen. The analysis of the activity of AC VI will be held by zymography. The determination of the concentration of CA VI in the biofilm will be performed by immunohistochemistry and activity of AC VI by Western blot. Salivary flow will be expressed in mL / min. The saliva pH will be measured with a commercial kit and specific electrode by the biofilm. The buffering capacity of saliva is measured eletrometrically. After 12 months, all procedures will be repeated to enable the evaluation of the study variables as predictors of caries increment in pre-school. Statistical analysis: The results will be submitted to the Wilcoxon test, Mann-Whitney and Spearman correlation (p = 0.05) and chi-square test followed by multiple logistic regression analysis to identify variables that may explain the development of dental caries after 01 year and the associations between the dependent and independent variables will be expressed as odds ratios (OR) with their respective confidence intervals of 95%. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BORGHI, GABRIELA N.; RODRIGUES, LIVIA P.; LOPES, LENITA M.; PARISOTTO, THAIS M.; STEINER-OLIVEIRA, CAROLINA; NOBRE-DOS-SANTOS, MARINES. Relationship among alpha amylase and carbonic anhydrase VI in saliva, visible biofilm, and early childhood caries: a longitudinal study. International Journal of Paediatric Dentistry, v. 27, n. 3, p. 174-182, . (12/15834-1)
RODRIGUES PICCO, DANIELE DE CASSIA; LOPES, LENITA MARANGONI; MARQUES, MARCELO ROCHA; PERES LINE, SERGIO ROBERTO; PARISOTTO, THAIS MANZANO; DOS SANTOS, MARINES NOBRE. Children with a Higher Activity of Carbonic Anhydrase VI in Saliva Are More Likely to Develop Dental Caries. Caries Research, v. 51, n. 4, p. 394-401, . (12/15834-1, 12/02886-3)
PICCO, DANIELE C. R.; MARANGONI-LOPES, LENITA; PARISOTTO, THAIS M.; MATTOS-GRANER, RENATA; NOBRE-DOS-SANTOS, MARINES. Activity of Carbonic Anhydrase VI is Higher in Dental Biofilm of Children with Caries. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 11, . (12/02886-3, 12/15834-1)

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