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Low-level laser therapy and topical sodium diclofenac in contusion-induced skeletal muscle injury in rats

Grant number: 12/06832-5
Support type:Regular Research Grants
Duration: March 01, 2013 - August 31, 2015
Field of knowledge:Engineering - Biomedical Engineering
Principal researcher:Lúcio Frigo
Grantee:Lúcio Frigo
Home Institution: Centro de Ciências Biológicas e da Saúde. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil
Assoc. researchers:Ernesto Cesar Pinto Leal Junior

Abstract

Among treatments employed to treat skeletal muscle injuries, the pharmacological therapy is the widely used possibly because its effects and mechanisms are sufficiently established. On the other hand, alternative therapies such low-level laser therapy (LLLT) still has some points to be clarified for complete evidence of your effects. With this perspective in mind, the aim of this project is to analyze and compare effects of sodium diclofenac (with topical application) and LLLT on biochemical, morphological and functional aspects when applied immediately after skeletal muscle trauma in rats. For such, will be performed morphological analysis employing histology (optical light microscopy), functional analysis employing measures of muscular strength and work in 3 electrical stimulation-induced tetanic contractions, and biochemical analysis employing quantification of gene expression (real-time polymerase chain reaction - RT-PCR) and protein expression (Western-Blott method) of Cyclooxygenase 1 and 2 enzymes (COX-1 and COX-2). Samples will be also analyszed employing imunoenzymatic test (ELISA) in order to quantify interleukins 1², 6 and 10 (IL-1², IL-6 and IL-10, respectivelly) and tumor necrosis factor alpha (TNF-±). (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE ALMEIDA, PATRICIA; TOMAZONI, SHAIANE SILVA; FRIGO, LUCIO; CAMILLO DE CARVALHO, PAULO DE TARSO; VANIN, ADRIANE AVER; SANTOS, LARISSA ALINE; ALBUQUERQUE-PONTES, GIANNA MOES; DE MARCHI, THIAGO; TAIROVA, OLGA; MARCOS, RODRIGO LABAT; et al. What is the best treatment to decrease pro-inflammatory cytokine release in acute skeletal muscle injury induced by trauma in rats: low-level laser therapy, diclofenac, or cryotherapy?. Lasers in Medical Science, v. 29, n. 2, SI, p. 653-658, . (10/52404-0, 12/06832-5)
TOMAZONI, SHAIANE SILVA; FRIGO, LUCIO; DOS REIS FERREIRA, TEREZA CRISTINA; CASALECHI, HELIODORA LEAO; TEIXEIRA, SIMONE; DE ALMEIDA, PATRICIA; MUSCARA, MARCELO NICOLAS; MARCOS, RODRIGO LABAT; SERRA, ANDREY JORGE; CAMILLO DE CARVALHO, PAULO DE TARSO; et al. Effects of photobiomodulation therapy and topical non-steroidal anti-inflammatory drug on skeletal muscle injury induced by contusion in rats-part 2: biochemical aspects. Lasers in Medical Science, v. 32, n. 8, p. 1879-1887, . (12/06832-5)
SANTOS, LARISSA ALINE; MARCOS, RODRIGO LABAT; TOMAZONI, SHAIANE SILVA; VANIN, ADRIANE AVER; ANTONIALLI, FERNANDA COLELLA; GRANDINETTI, VANESSA DOS SANTOS; ALBUQUERQUE-PONTES, GIANNA MOES; VICENTE DE PAIVA, PAULO ROBERTO; BRANDAO LOPES-MARTINS, RODRIGO ALVARO; CAMILLO DE CARVALHO, PAULO DE TARSO; et al. Effects of pre-irradiation of low-level laser therapy with different doses and wavelengths in skeletal muscle performance, fatigue, and skeletal muscle damage induced by tetanic contractions in rats. Lasers in Medical Science, v. 29, n. 5, p. 1617-1626, . (10/52404-0, 12/04295-2, 12/06832-5)
ALBUQUERQUE-PONTES, GIANNA MOES; VIEIRA, RODOLFO DE PAULA; TOMAZONI, SHAIANE SILVA; CAIRES, CLAUDIA OLIVEIRA; NEMETH, VICTORIA; VANIN, ADRIANE AVER; SANTOS, LARISSA ALINE; PINTO, HENRIQUE DANTAS; MARCOS, RODRIGO LABAT; BJORDAL, JAN MAGNUS; et al. Effect of pre-irradiation with different doses, wavelengths, and application intervals of low-level laser therapy on cytochrome c oxidase activity in intact skeletal muscle of rats. Lasers in Medical Science, v. 30, n. 1, p. 59-66, . (12/11385-8, 10/52404-0, 12/06832-5)
PINTO LEAL-JUNIOR, ERNESTO CESAR; DE ALMEIDA, PATRICIA; TOMAZONI, SHAIANE SILVA; CAMILLO DE CARVALHO, PAULO DE TARSO; BRANDAO LOPES-MARTINS, RODRIGO ALVARO; FRIGO, LUCIO; JOENSEN, JON; JOHNSON, MARK I.; BJORDAL, JAN MAGNUS. Superpulsed Low-Level Laser Therapy Protects Skeletal Muscle of mdx Mice against Damage, Inflammation and Morphological Changes Delaying Dystrophy Progression. PLoS One, v. 9, n. 3, . (10/52404-0, 12/06832-5)
TOMAZONI, SHAIANE SILVA; FRIGO, LUCIO; DOS REIS FERREIRA, TEREZA CRISTINA; CASALECHI, HELIODORA LEO; TEIXEIRA, SIMONE; DE ALMEIDA, PATRICIA; MUSCARA, MARCELO NICOLAS; MARCOS, RODRIGO LABAT; SERRA, ANDREY JORGE; DE CARVALHO, PAULO DE TARSO CAMILLO; et al. Effects of photobiomodulation therapy and topical non-steroidal anti-inflammatory drug on skeletal muscle injury induced by contusion in rats-part 1: morphological and functional aspects. Lasers in Medical Science, v. 32, n. 9, p. 2111-2120, . (12/06832-5)

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