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Analysis of the lipodemic profile and bioavailability of natural and bio-transformed flavonoids in rats

Grant number: 12/20393-4
Support Opportunities:Regular Research Grants
Duration: March 01, 2013 - August 31, 2015
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Patrícia de Oliveira Carvalho
Grantee:Patrícia de Oliveira Carvalho
Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil


Earlier reports on our research group have shown that hydrolysis of the glycoside fraction of rutin enhance its antioxidant and anti-proliferative activities as measured by in vitro tests. Enzymatic hydrolysis with total or partial liberation of the glycosidic fraction is capable of generating simpler compounds with enhanced functionality. This study is designed to make comparative assessments of the effects of two flavonoids (rutin and hesperidin) on the plasma lipids profile and the biochemical parameters involved in the oxidative processes before and after enzymatic modification. For test purposes, the compounds (1mmol eq flavonoid/Kg animal live weight) will be administered orally to male Wistar rats for 30 days. Plasma lipids, erythrocytes and tissues will be extracted using chloroform and methanol, the classes of phospholipids separated by solid phase extraction (SPE) chromatography and the fatty acids composition determined by gas chromatography (GC). Plasma concentration of aglycone will be analyzed using ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS). Additionally, biochemical-molecular procedures will be undertaken to quantify malondialdehyde, antioxidant enzymes and expressions of desaturases (FADS1 and FADS2) in the animals' plasma and tissues. This proposal is intended to contribute to obtaining greater understanding of the effects of flavonoids and their hydrolyzed derivatives and to elucidate those effects in the light of the compounds' structures. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DINI DE FRANCO, ELIDA PAULA; CONTESINI, FABIANO JARES; DA SILVA, BIANCA LIMA; ALVES DE PILOTO FERNANDES, ANNA MARIA; LEME, CAMILA WIELEWSKI; GONCALVES CIRINO, JOAO PEDRO; BUENO CAMPOS, PAULA RENATA; CARVALHO, PATRICIA DE OLIVEIRA. Enzyme-assisted modification of flavonoids from Matricaria chamomilla: antioxidant activity and inhibitory effect on digestive enzymes. Journal of Enzyme Inhibition and Medicinal Chemistry, v. 35, n. 1, p. 42-49, . (12/20393-4)
DE SOUZA, VERONICA TRICOLI; DINI DE FRANCO, ELIDA PAULA; MELO BRANCO DE ARAUJO, MARIA ELISA; FERNANDES MESSIAS, MARCIA CRISTINA; MARINHO PRIVIERO, FERNANDA BRUSCHI; FRANKLAND SAWAYA, ALEXANDRA C. H.; CARVALHO, PATRICIA DE OLIVEIRA. Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin: an in vitro and in vivo study. JOURNAL OF MOLECULAR RECOGNITION, v. 29, n. 2, p. 80-87, . (12/11050-6, 12/20393-4)

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