Association between SNP related to adiponectin, C-reactive protein, Toll Like Receptor 4, TNF-alfa, IL-1 beta, IL-6 and IL-10 genes and lipids intake and their effects on plasma inflammatory biomarkers levels in population-based study

Abstract

The nutritional transition is defined by economic, social, demographic and behavior changes, which interfere directly with a population dietary pattern. Latest years, technological revolution contributed to a grown availability of food products rich in saturated fatty acids, trans fatty acids, and polyunsaturated n-6 fatty acids and poor in dietary fiber, vitamins and minerals. The quantity and quality of dietary fatty acids have won a prominent role in studies involving nutritional genomic because these nutrients modulate the eicosanoid synthesis and the activation of intracellular signaling pathways related to the inflammatory response. Furthermore, it is worth mentioning that genetic variations can interfere with the interaction of dietary fatty acids and inflammatory biomarkers. This study aims to verify the association between SNP related to adiponectin, C-reative protein, Toll like receptor (TLR) 4, tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6 and IL-10 and lipids intake and their effects on plasma inflammatory biomarkers levels in a population-based study in the city of São Paulo. Three-hundred and three adult subjects will be studied, aging between 20 and 59 years old. The method used to collect dietary data was 24-hours recall, applied in duplicate. The dietary data were transformed in energy and nutrients using Nutrition Data System for Research program (NDS-R, Minessota, USA). The domiciliary blood collection was performed by specialized professionals, after 12 hours fasting, with previous scheduling. The levels of IL-1 beta, IL-6, IL-8, IL-10, IL-12p70, TNF-alpha, adiponectin, C-reactive protein, macrophage chemotactic protein (MCP)-1, soluble intracellular adhesion molecule (sICAM)-1 and soluble vascular cell adhesion molecule (sVCAM)-1 will be determine from plasma with immunoassay multiplex technique. The genomic DNA will be extracted from blood using salting out method and the SNP genotyping of the following genes: adiponectin (rs266729, rs17300539, rs16861209, rs1501299 and rs2241766), C-reactive protein (rs1205, rs1417938, rs1800947, rs2808630 and rs3093059), TLR4 (rs4986790 and rs5030728), TNF-alpha (rs1799964, rs1799724, rs1800629 and rs361525), IL-1 beta (rs1143623, rs16944, rs1143627, rs1143634 and rs1143643), IL-6 (rs1800795) and IL-10 (rs1800871, rs1800892 and rs1800872) will be made by specific allele PCR technique. (AU)