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Proteomic and peptidomic characterization of Brazilian spider venoms by mass spectrometry


The diversity of biological activities found in spider secretions as venom and hemolymph have motivated several research groups to search for molecules with potential therapeutic use, which results in an increasing number of toxins isolated and sequenced over the years. But despite advances, the number of spider peptides isolated and characterized is estimated to be only 0.01% of all possible existing sequences. Recent studies have indicated the presence of components with antimicrobial activity in the venom of various Brazilian spiders. However, few of these components have been characterized. Considering the biological potential of molecules present in the venoms, the importance of characterizing these toxins and the importance of exploring new active molecules, we propose in this research project the fractionation of proteins and peptides present in the venom of Brazilian spiders, identification of biological activities and determination of their primary structures by mass spectrometry analysis and de novo sequencing. (AU)

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Scientific publications (14)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEDROSO, AMANDA P.; DORNELLAS, ANA P. S.; DE SOUZA, ADRIANA P.; PAGOTTO, JOSIAS F.; OYAMA, LILA M.; NASCIMENTO, CLAUDIA M. O.; KLAWITTER, JELENA; CHRISTIANS, UWE; TASHIMA, ALEXANDRE K.; RIBEIRO, ELIANE BERALDI. A proteomics-metabolomics approach indicates changes in hypothalamic glutamate-GABA metabolism of adult female rats submitted to intrauterine growth restriction. EUROPEAN JOURNAL OF NUTRITION, v. 58, n. 8, p. 3059-3068, . (12/19321-9, 10/20268-0)
DASGUPTA, SAYANI; YANG, CIYU; CASTRO, LEANDRO M.; TASHIMA, ALEXANDRE K.; FERRO, EMER S.; MOIR, ROBYN D.; WILLIS, IAN M.; FRICKER, LLOYD D.. Analysis of the Yeast Peptidome and Comparison with the Human Peptidome. PLoS One, v. 11, n. 9, . (12/19321-9)
PEDROSO, AMANDA P.; SOUZA, ADRIANA P.; DORNELLAS, ANA P. S.; OYAMA, LILA M.; NASCIMENTO, CLAUDIA M. O.; SANTOS, GIANNI M. S.; ROSA, JOSE C.; BERTOLLA, RICARDO P.; KLA-WITTER, JELENA; CHRISTIANS, UWE; et al. Intrauterine Growth Restriction Programs the Hypothalamus of Adult Male Rats: Integrated Analysis of Proteomic and Metabolomic Data. JOURNAL OF PROTEOME RESEARCH, v. 16, n. 4, p. 1515-1525, . (12/19321-9, 10/20268-0)
TASHIMA, ALEXANDRE KEIJI; CASTILHO, DANIELE GONCALVES; ALENCAR CHAVES, ALISON FELIPE; XANDER, PATRICIA; ZELANIS, ANDRE; BATISTA, WAGNER LUIZ. Data in support of quantitative proteomics to identify potential virulence regulators in Paracoccidioides brasiliensis isolates. DATA IN BRIEF, v. 5, p. 155-160, . (14/13961-1, 12/19321-9, 11/14392-2)
MORGON, ADRIANO M.; BELISARIO-FERRARI, MATHEUS R.; TREVISAN-SILVA, DILZA; MEISSNER, GABRIEL O.; VUITIKA, LARISSA; MARIN, BRENDA; TASHIMA, ALEXANDRE K.; GREMSKI, LUIZA H.; GREMSKI, WALDEMIRO; SENFF-RIBEIRO, ANDREA; et al. Expression and immunological cross-reactivity of LALP3, a novel astacin-like metalloprotease from brown spider (Loxosceles intermedia) venom. Biochimie, v. 128, p. 8-19, . (12/19321-9)
DE MORAIS-ZANI, KAREN; SERINO-SILVA, CAROLINE; GALIZIO, NATHALIA DA COSTA; TASIMA, LIDIA JORGE; PAGOTTO, JOSIAS FALARARO; TEIXEIRA DA ROCHA, MARISA MARIA; MARCELINO, JOSE ROBERTO; SANT'ANNA, SAVIO STEFANINI; TASHIMA, ALEXANDRE KEIJI; TANAKA-AZEVEDO, ANITA MITICO; et al. Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?. JOURNAL OF PROTEOMICS, v. 174, p. 17-27, . (14/11108-0, 16/09976-9, 17/01890-0, 12/19321-9)
CASTILHO, DANIELE G.; CHAVES, ALISON F. A.; XANDER, PATRICIA; ZELANIS, ANDRE; KITANO, EDUARDO S.; SERRANO, SOLANGE M. T.; TASHIMA, ALEXANDRE K.; BATISTA, WAGNER L.. Exploring Potential Virulence Regulators in Paracoccidioides brasiliensis Isolates of Varying Virulence through Quantitative Proteomics. JOURNAL OF PROTEOME RESEARCH, v. 13, n. 10, p. 4259-4271, . (12/19321-9, 11/14392-2)
ABREU, THIAGO F.; SUMITOMO, BIANCA N.; NISHIYAMA, JR., MILTON Y.; OLIVEIRA, URSULA C.; SOUZA, GUSTAVO H. M. F.; KITANO, EDUARDO S.; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.; JUNQUEIRA-DE-AZEVEDO, INACIO; SILVA, JR., PEDRO I.; et al. Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 232-242, . (12/19321-9)
TASHIMA, ALEXANDRE K.; FRICKER, LLOYD D.. Quantitative Peptidomics with Five-plex Reductive Methylation labels. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, v. 29, n. 5, p. 866-878, . (12/19321-9)
CHAVES, ALISON F. A.; CASTILHO, DANIELE G.; NAVARRO, MARINA V.; OLIVEIRA, ANA K.; SERRANO, SOLANGE M. T.; TASHIMA, ALEXANDRE K.; BATISTA, WAGNER L.. Phosphosite-specific regulation of the oxidative-stress response of Paracoccidioides brasiliensis: a shotgun phosphoproteomic analysis. Microbes and Infection, v. 19, n. 1, p. 34-46, . (14/13961-1, 11/14392-2, 13/07467-1, 12/19321-9, 12/17530-0)
DASGUPTA, SAYANI; FISHMAN, MICHAEL A.; MAHALLATI, HANA; CASTRO, LEANDRO M.; TASHIMA, ALEXANDRE K.; FERRO, EMER S.; FRICKER, LLOYD D.. Reduced Levels of Proteasome Products in a Mouse Striatal Cell Model of Huntington's Disease. PLoS One, v. 10, n. 12, . (12/19321-9)
JOCELINO GOMES DE LACERDA, JOSE THALLES; RODRIGUES E LACERDA, RODRIGO; ASSUNCAO, NILSON ANTONIO; TASHIMA, ALEXANDRE KEIJI; JULIANO, MARIA APARECIDA; DOS SANTOS, JR., GILBERTO ALVES; DE SOUZA, MILENY DOS SANTOS; BATISTA, JACINTO DE LUNA; ROSSI, CARLOS EDUARDO; DE ALMEIDA GADELHA, CARLOS ALBERTO; et al. New insights into lectin from Abelmoschus esculentus seeds as a Kunitz-type inhibitor and its toxic effects on Ceratitis capitata and root-knot nematodes Meloidogyne spp.. Process Biochemistry, v. 63, p. 96-104, . (12/19321-9, 12/02514-9, 12/50191-4)
SERINO-SILVA, CAROLINE; MORAIS-ZANI, KAREN; TOYAMA, MARCOS HIKARI; TOYAMA, DANIELA DE OLIVEIRA; GAETA, HENRIQUE HESSE; BITTENCOURT RODRIGUES, CAROLINE FABRI; AGUIAR, WESLEI DA SILVA; TASHIMA, ALEXANDRE KEIJI; GREGO, KATHLEEN FERNANDES; TANAKA-AZEVEDO, ANITA MITICO. Purification and characterization of the first gamma-phospholipase inhibitor (gamma PLI) from Bothrops jararaca snake serum. PLoS One, v. 13, n. 3, . (14/11108-0, 13/12826-0, 17/01890-0, 17/16908-2, 12/19321-9, 13/05357-4)

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