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Characterization of the vaginal microbiome of Brazilian women in reproductive age

Abstract

Studies on the vaginal microbiome have started only recently, following the researches for characterizing the human microbiome. Describing the real composition of microbial community in the vagina by microbiome studies is crucial for women's health, since abnormal vaginal microbiota is associated with poor pregnancy outcome and increased risk for acquisition of sexually transmitted infections. Up to now, the most important study on vaginal micobiome was performed on samples from North-American women. It was demonstrated that although the vaginal microbiota is variable and dynamic, it could be placed into one of 5 communities, according to the types and abundance of the species present. The major part of the vaginal communities from women experiencing bacterial vaginosis (BV) is placed in one of the 5 types of communities that is characterized by lower load of lactobacillus species. This community can be divided in 3 sub-communities, according to the interactions among certain bacterial species. To date, no study has been performed aiming to determine the vaginal microbiome of Brazilian women. Considering the heterogeneity on hygiene practices and sexual behavior, as well as the high prevalence of gynecological and obstetrical complications among Brazilian women, the objective of this study is to characterize the vaginal microbiome of Brazilian women from the five regions of the country. Bacterial species and their abundance will be determined by pyrosequencing a region of the 16S rRRNA gene. Classification of the vaginal microbiota will be also performed according to Nugent's criteria in Normal, Intermediate and BV, on Gram-stained smears. Sub-communities of bacterial species will be assessed on cases of BV. Total bacterial cunt by flux cytometry will be performed on vaginal samples from women presenting bacterial vaginosis. Samples will be also submitted to correlation tests between presence of certain sub-communities and total bacterial count, when BV is detected. Thus, these results will allow the first-time description of the vaginal microbiome in Brazilian women and may contribute to future strategies for improving women's reproductive health. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA, CAROLINA SANITA TAFNER; MARCONI, CAMILA; PARADA, CRISTINA M. G. L.; RAVEL, JACQUES; SILVA, MARCIA GUIMARAES DA. ialidase Activity in the Cervicovaginal Fluid Is Associated With Changes in Bacterial Components of Lactobacillus-Deprived Microbiot. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, . (12/10403-2, 12/16800-3)
TAFNER FERREIRA, CAROLINA SANITA; DONDERS, GILBERT GERARD; MARCIA DE LIMA PARADA, CRISTINA MARIA; TRISTAO, ANDREA DA ROCHA; FERNANDES, THAIZ; DA SILVA, MARCIA GUIMARAES; MARCONI, CAMILA. Treatment failure of bacterial vaginosis is not associated with higher loads of Atopobium vaginae and Gardnerella vaginalis. Journal of Medical Microbiology, v. 66, n. 8, p. 1217-1224, . (12/16800-3, 12/10403-2)
NOVAK, JULIANO; RAVEL, JACQUES; MA, BING; TAFNER FERREIRA, CAROLINA SANITA; TRISTAO, ANDREA DA ROCHA; SILVA, MARCIA GUIMARAES; MARCONI, CAMILA. Characteristics associated with Lactobacillus iners-dominated vaginal microbiota. SEXUALLY TRANSMITTED INFECTIONS, . (12/16800-3)
NODA-NICOLAU, NATHALIA MAYUMI; SILVA, MARIANA DE CASTRO; COSI BENTO, GIOVANA FERNANDA; BAPTISTA FERREIRA, JENIFFER SENA; NOVAK, JULIANO; PESSOA MORALES, JULIA ANDRADE; TRONCO, JULIA ABBADE; BOLPETTI, ALINE NASCIMENTO; SILVA PINTO, GABRIEL VITOR; POLETTINI, JOSSIMARA; et al. Cervicovaginal levels of human beta defensins during bacterial vaginosis. PLoS One, v. 16, n. 12, . (12/16800-3)
MARCONI, CAMILA; EL-ZEIN, MARIAM; RAVEL, JACQUES; MA, BING; LIMA, MOISES D.; CARVALHO, NEWTON SERGIO; ALVES, ROSANE R. F.; PARADA, CRISTINA M. G. L.; LEITE, SANDRA H. M.; GIRALDO, PAULO C.; et al. Characterization of the Vaginal Microbiome in Women of Reproductive Age From 5 Regions in Brazil. SEXUALLY TRANSMITTED DISEASES, v. 47, n. 8, p. 562-569, . (12/10403-2, 14/22463-5, 12/16800-3)
LUCHIARI, HELOISE R.; FERREIRA, CAROLINA S. T.; GOLIM, MARJORIE A.; SILVA, MARCIA G.; MARCONI, CAMILA. Cervicovaginal bacterial count and failure of metronidazole therapy for bacterial vaginosis. International Journal of Gynecology & Obstetrics, v. 132, n. 3, p. 297-301, . (12/16800-3, 12/10403-2, 13/01750-3)

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