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Collagen imobilization on poly (L-co-D, L lactic acid) scaffolds

Abstract

Bioreabsorbable polymers are widely used as scaffolds in tissue engineering. However, due to their hydrophobic nature, modification techniques based on graft of chemical functional groups and immobilization of bioactive molecules have been developed, in order to promote biofuncionalization of material surface, causing better interaction between cell / polymer. This study aim at developing and characterizing a biomimetic surface on scaffolds of the copolymer poly (L-co-D, L lactic acid) (PLDLA 70/30), by grafting of chemical functional groups and immobilized with collagen type I. The scaffolds will be obtained by porogen leaching, and submitted to photo-oxidation method of the material surface followed by grafting polymer polymerization of the acrylic acid solution (PLDLA-Acc) and immobilization of the type I collagen (PLDLA-Col). The surface sample will be characterized before and after surface modification treatment by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Attenuated Total Reectance Infrared Spectroscopy (ATR-FTIR), X-ray Photoelectron Spectroscopy (XPS) and contact angle. Scaffolds cytocompatibilty will be evaluated by osteoblastic-like cell culture, submitted to cytotoxicity and initial cell adhesion assays, collagen synthesis and observation of cell morphology obtained by SEM. The biocompatibility of PLDLA-Col scaffolds will be evaluated by material implant into critical-size defect (8mm) carried out on calvarial Wistar rats. After 8 and 12 week of implant the area of new bone formed will be evaluated by histologically and histomorphometry assay. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MAS, BRUNA ANTUNES; DE LUNA FREIRE, DIEGO COUTINHO; DE MELO CATTANI, SILVIA MARA; MOTTA, ADRIANA CRISTINA; PERIS BARBO, MARIA LOURDES; DE REZENDE DUEK, ELIANA APARECIDA. Biological Evaluation of PLDLA Polymer Synthesized as Construct on Bone Tissue Engineering Application. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 19, n. 2, p. 300-307, MAR-APR 2016. Web of Science Citations: 2.
MAS, BRUNA ANTUNES; DE MELLO CATTANI, SILVIA MARA; CIPRIANO RANGEL, RITA DE CASSIA; RIBEIRO, GABRIELLE DE ALMEIDA; CRUZ, NILSON CRISTINO; LEITE, FABIO DE LIMA; DE PAULA NASCENTE, PEDRO AUGUSTO; DE REZENDE DUEK, ELIANA APARECIDA. Surface Characterization and Osteoblast-like Cells Culture on Collagen Modified PLDLA Scaffolds. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 17, n. 6, p. 1523-1534, NOV-DEC 2014. Web of Science Citations: 6.
BRUNA ANTUNES MÁS; SILVIA MARA DE MELLO CATTANI; RITA DE CÁSSIA CIPRIANO RANGEL; GABRIELLE DE ALMEIDA RIBEIRO; NILSON CRISTINO CRUZ; FABIO DE LIMA LEITE; PEDRO AUGUSTO DE PAULA NASCENTE; ELIANA APARECIDA DE REZENDE DUEK. Surface characterization and osteoblast-like Cells culture on collagen modified PLDLA scaffolds. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 17, n. 6, p. 1523-1534, 2014-12-00.

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