Influence of adiposity in inflammation, oxidation and adipocytokies in breast cancer

Abstract

The prevalence of cancer has increased in recent decades and, thus, cancer remains a serious public health problem worldwide. Among the different types of cancer, breast cancer represents worldwide the second most common form. In Brazil were estimated for 2010 and 2011 almost 50 000 new cases of breast cancer. The etiology of breast cancer could be partly related to oxidative stress where several mechanisms promote oxidative reactions and irreversible damage to DNA. Excess weight and adipose tissue plays an important role in tumor development and clinical prognosis of breast cancer, because of its characteristic inflammatory, oxidative and endocrine. Objective: To evaluate the role of fat in inflammation, oxidation and adipocytokines in breast cancer. Methods: The project consists of an analytical, observational, cross-sectional, which will be evaluated two groups: Group 1 (Case) - Women with breast cancer, and Group 2 (Control) - women without breast cancer. Eligible patients will be treated in the department mastology General Hospital of Fortaleza - HGF (COEP: 050507/10). In the first group are included patients with clinical stage I, II and III without associated neoplasia, without prior anticancer treatment and Karnofsky performance status> 70. In group 2, patients will be included without a diagnosis of breast cancer or any other cancer. Will be collected in structured form socio-economic and clinical. To determine the nutritional status will be collected weight, height and waist circumference. The assessment of body composition will be performed by bioelectrical impedance. For biochemical analysis will be collected 20ml of blood after 12h of fasting. I-will be evaluated biomarkers of inflammation (IL6, TNF-alpha, CRP), II-oxidative biomarkers (Malondialdehyde, MDA and conjugated diene, LDL-detection and auto-anti-LDL-) III - Oxidative damage to DNA ( 8-hydroxy-2'-deoxyguanosine - 8-OHdG), IV - exogenous antioxidants (antioxidant vitamins) and V - Concentration of adipocytokines (leptin and adiponectin). Analysis of results - The results will be evaluated with the aid of the Statistical Package for Social Sciences (SPSS) version 15.0. Regression models are linear and logistic univariate and multivariate analysis, which will assess the relationship between BMI, WC and fat percentage (independent variables) and oxidative parameters, and inflammatory adipocytokines (dependent variables). In addition to assessing the interaction between variables, women, after stratification by BMI, WC and fat percentage will be compared using parametric tests (t-test) or nonparametric (Mann-Whitney test). The distribution of the variables will be evaluated using the Kolmogorov-Smirnov and the value of significance is p <0.05. (AU)