Anthony e beezer | city university/londres - Inglaterra

Abstract

Calorimetry plays an important role in biotechnology. Determination of the kinetics and thermodynamics of reactions of biological molecules is necessary for understanding the structures and functions of these molecules, and in bioengineering living systems, and for making pharmaceutical preparations. All biochemical reactions involve recognition, binding and the formation of non-covalent complexes. Since the characterization of binding events is central to understanding any physiological process at the molecular level, biochemical and biomedical research requires methodologies for precisely analyzing binding reactions. Isothermal titration calorimetry (ITC) can provide a full thermodynamic characterization of an interaction.The widespread availability of isothermal titration calorimeters has revolutionized the study of biochemistry. The recent development of high-sensitivity instruments allowing rapid, accurate thermodynamic characterization of binding events has provided a new level of information with which to attempt to understand biomolecular interactions: Protein-small molecule interactions, protein-drug/inhibitor interactions, protein-carbohydrate interactions, protein folding, along with, detection of protonation, investigation of micelles, drug design, and thermodynamic-structure correlation are among the extensive applications of calorimetry. Professor Beezer has a long story and skill in many areas of calorimetry: application of chemometric analysis to complexity in isothermal calorimetric data, development of an isothermal heat-conduction photocalorimeter, prediction of seed longevity, membrane transport and implications for dermal delivery, stability assessment of pharmaceuticals and so on. The calorimetry expertise needed for efficient access to the data generated in our laboratory will be much enhanced with his visit. The goals of the visit are to accomplish with: mathematics of the Michaelis-Menten kinetics, modeling of the dilution processes in multiple injection systems, protocol advance for host-guest interaction, short course on biothermodynamics and the state-of-art of calorimetry in drug formulation. (AU)