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Quantitative and molecular evaluation of a continuous crossbreeding system using Oreochromis niloticus Chitralada and red stirling strains

Grant number: 11/23752-2
Support Opportunities:Regular Research Grants
Duration: July 01, 2012 - June 30, 2015
Field of knowledge:Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
Principal Investigator:Alexandre Wagner Silva Hilsdorf
Grantee:Alexandre Wagner Silva Hilsdorf
Host Institution: Pró-Reitoria de Pesquisa, Pós-Graduação e Extensão. Universidade de Mogi das Cruzes (UMC). Mogi das Cruzes , SP, Brazil
Associated researchers: Aline de Assis Lago ; Ivan Bezerra Allaman ; Rilke Tadeu Fonseca de Freitas

Abstract

The tilápia Oreochromis niloticus is widely distributed fish species in Brazil and is the most farmed among the Brazilian freshwater fishes. Currently, few programs on genetic breeding have been carried out in Brazil. Aiming at producing a tilápia strain that put together superior performance and red coloration, the present project intends to apply a breeding program using the Chitralada and the Red Stirling strains as well as to use molecular markers to monitor the crossbreeding, and identify possible GH markers to assist selection. The project will be done at the "Indústria Brasileira do Peixes Ltda." hatchery in Jundiaí (SP) and in the "Laboratório de Genética de Organismos Aquáticos e Aquicultura" (LAGOAA) at the University of Mogi das Cruzes (SP). Genetic variability analysis using microsatellites will be carried out with the parental strains and throughout the crossings. Crossbreeding will be done from 1/2 Chitralada and 1/2 Red Stirling to produce 3/4 Chitralada and 1/4 Red Stirling and then after blotching free culling the 7/8 Chitralada en 1/8 Red Stirling progeny. Simultaneously, intra-crossings within Chitralada and Red-Stirling parental strains will be done to produce contemporary progenies to compare to the crossbreedings. Data of farming performance will be recorded all over the progeny testing. Fin clipping of the crossbreeding progeny will be taken for DNA extraction to assess the polymorphism in the Intron I and promoter of the Growth Hormone Gene and associate it to the growth performance. The present proposal endeavors to develop methodologies for the tilapia genetic breeding and also to be applied to other Brazilian native farmed fish species. (AU)

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Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PERAZZA, CAIO AUGUSTO; VALERIO PINAFFI, FABIO LUIS; SILVA, LUCIANO ANDRADE; SILVA HILSDORF, ALEXANDRE WAGNER. EVALUATION OF ULTRASOUND IMAGING TO PREDICT LOIN EYE AREA IN TAMBAQUI. Boletim do Instituto de Pesca, v. 41, n. SI, p. 803-809, . (11/23752-2)
DIAS, M. A. D.; DE FREITAS, R. T. F.; ARRANZ, S. E.; VILLANOVA, G. V.; HILSDORF, A. W. S.. Evaluation of the genetic diversity of microsatellite markers among four strains of Oreochromis niloticus. ANIMAL GENETICS, v. 47, n. 3, p. 345-353, . (11/23752-2)
DESSIMONI DIAS, MARCO AURELIO; REIS NETO, RAFAEL VILHENA; DE SOUSA BUENO FILHO, JULIO SILVIO; KHALIL JASER, SUHAILA KARIM; LAGO, ALINE DE ASSIS; SILVA HILSDORF, ALEXANDRE WAGNER. Growth hormone gene polymorphism associated with grow-out performance of Oreochromis niloticus strains. Aquaculture, v. 503, p. 105-110, . (11/23752-2)
HERKENHOFF, MARCOS EDGAR; BOVOLENTA, LUIZ A.; BROEDEL, OLIVER; DOS SANTOS, LUCILENE D.; DE OLIVEIRA, ARTHUR C.; CHUFFA, LUIZ G. A.; RIBEIRO, AMANDA DE O.; LUPI, LUIZ A.; DIAS, MARCO A. D.; HILSDORF, ALEXANDRE W. S.; et al. ariant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus. Genomics, v. 113, n. 6, p. 4303-4312, . (14/03062-0, 11/23752-2)
LAGO, ALINE DE ASSIS; REIS-NETO, RAFAEL VILHENA; REZENDE, TIAGO TERUEL; DA SILVA RIBEIRO, MAYRA CRISTINA; FONSECA DE FREITAS, RILKE TADEU; SILVA HILSDORF, ALEXANDRE WAGNER. Quantitative analysis of black blotching in a crossbred red tilapia and its effects on performance traits via a path analysis methodology. JOURNAL OF APPLIED GENETICS, v. 60, n. 3-4, p. 393-400, . (11/23752-2)
FONSECA, FERNANDO S.; DOMINGUES, RODRIGO R.; HALLERMAN, ERIC M.; HILSDORF, ALEXANDRE W. S.. Genetic Diversity of an Imperiled Neotropical Catfish and Recommendations for Its Restoration. FRONTIERS IN GENETICS, v. 8, . (11/23752-2)
LAGO, ALINE DE ASSIS; REZENDE, TIAGO TERUEL; DESSIMONI DIAS, MARCO AURELIO; FONSECA DE FREITAS, RILKE TADEU; SILVA HILSDORF, ALEXANDRE WAGNER. The development of genetically improved red tilapia lines through the backcross breeding of two Oreochromis niloticus strains. Aquaculture, v. 472, n. S, SI, p. 17-22, . (11/23752-2)
JULIANA BELTRAMIN DE BIASI; ACÁCIO RIBEIRO GOMES TOMÁS; ALEXANDRE WAGNER SILVA HILSDORF. Molecular evidence of two cryptic species of Stramonita (Mollusca, Muricidae) in the southeastern Atlantic coast of Brazil. GENETICS AND MOLECULAR BIOLOGY, n. ahead, p. 0-0, . (11/23752-2)
JULIANA BELTRAMIN DE BIASI; ACÁCIO RIBEIRO GOMES TOMÁS; ALEXANDRE WAGNER SILVA HILSDORF. Molecular evidence of two cryptic species of Stramonita (Mollusca, Muricidae) in the southeastern Atlantic coast of Brazil. GENETICS AND MOLECULAR BIOLOGY, v. 39, n. 3, p. 392-397, . (11/23752-2)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.