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Mitochondrial function during adipogenesis in the white adipose tissue (WAT): function of fatty acids


Mitochondrial biogenesis and metabolism are involved in the regulation of white adipose tissue functioning, such as adipogenesis, lipid and carbohydrate metabolism, preadipocyte proliferation, adipocyte dedifferentiation and triglyceride accumulation. However, this issue is still under debate. The goal of this project is to investigate the effect of saturated and insaturated fatty acids (FA) on mitochondrial metabolism and their implication on preadipocytes differentiation into mature adipocytes (adipogenesis). The clonal cell line 3T3-L1 will be employed, as well as primary preadipocytes driven to the differentiation into mature adipocytes. These cells will be treated in different periods of cell culture with the following FA: palmitic (C16:0), palmitoleic (C16:1 omega-7), linoleic (C18:2, omega-6), eicosapentaenoic (EPA, C20:5 omega-3) and docosahexaenoic (DHA, C22:6 omega-3). The following parameters will be evaluated: (1) mitochondrial biogenesis and metabolism, (2) adipogenic potential of primary preadipocytes and 3T3-L1 cells, (3) peroxisome proliferator-activated receptor (PPAR)-y coactivator (PGC)-1a, transcription factor A (TFAM), carnitine palmitoyl transferase 1 (CPT1), nuclear respiratory factor 1 (NRF1), uncoupling protein 2 (UCP2) and manganese superoxide dismutase (MnSOD) gene expression, and (4) mitochondrial proteomics analysis during adipogenesis. The following techniques will be employed: fluorimetry, lumínescence, real time RT-PCR, western blotting and proteomics. With the results obtained we will contribute to the elucidation of the role of mitochondria and different FA in adipogenesis. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CRUZ, MAYSA M.; LOPES, ANDRESSA B.; CRISMA, AMANDA R.; DE SA, ROBERTA C. C.; KUWABARA, WILSON M. T.; CURI, RUI; DE ANDRADE, PAULA B. M.; ALONSO-VALE, MARIA I. C.. Palmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes. LIPIDS IN HEALTH AND DISEASE, v. 17, . (11/51627-8, 11/51701-3)

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