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Functional analysis of cell signaling involvement in cancer stem cells obtained from oral squamous cell carcinoma cell lines

Grant number: 12/00786-1
Support Opportunities:Regular Research Grants
Duration: June 01, 2012 - November 30, 2014
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Fabio Daumas Nunes
Grantee:Fabio Daumas Nunes
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers:Camila de Oliveira Rodini Pegoraro ; Maria Fernanda Setúbal Destro Rodrigues


Oral squamous cell carcinoma is the most prevalent neoplasia of oral cavity. The comprehension of this neoplasia is limited regarding its origin and which cells are involved in tumor development and progression, becoming a challenge to the establishment of effective treatment and preventive strategies. In view of this, the identification of neoplastic cells subpopulations on oral squamous cell carcinoma that present the tendency to tumoral expansion, metastasis and alteration in cell death pathways is essential to treatment improvement and survival rates increase of oral squamous cell carcinoma patients. Recently, the discovery that head and neck squamous cell carcinoma comprise a subpopulation of tumoral initiators cells that apparently correspond to malignant stem cells responsible for tumor growth arose the hypothesis of new therapeutic perspectives, as well as the understanding of tumor development mechanisms. Regardless advances on stem cells research, there are few studies focused on the analysis of oral squamous cell carcinoma stem cells. The main purpose of the present study is the isolation and characterization of oral squamous cell carcinoma stem cells from established cell lines using specifics cell molecules. Additionally, it will be analyzed the gene expression profile of genes related to important cell signaling pathways and involved in the regulation of stem cells, and also, their functional hole in controlling essentials cellular processes to malignant transformation contributing to the comprehension of the events involved in the development and evolution of this tumor. Then, diagnostic and therapeutic approaches might become possible after the discovery of potential molecular targets of oral squamous cell carcinoma. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; TOBOUTI, PRISCILA LIE; MOLON, ANGELA CRISTINA; SEDASSARI, BRUNO TAVARES; NUNES, FABIO DAUMAS; PINTO JUNIOR, DECIO DOS SANTOS; ORSINI MACHADO DE SOUSA, SUZANA CANTANHEDE. Histopathological findings and immunohistochemical expression of the stem cell markers CD44, ALDH1, Bmi-1, and Nanog in oral solitary fibrous tumors. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 131, n. 4, p. 444-451, . (11/21395-8, 12/00786-1)
DE ANDRADE, NATHALIA PAIVA; SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; RODINI, CAMILA OLIVEIRA; NUNES, FABIO DAUMAS. Cancer stem cell, cytokeratins and epithelial to mesenchymal transition markers expression in oral squamous cell carcinoma derived from ortothopic xenoimplantation of CD44(high) cells. PATHOLOGY RESEARCH AND PRACTICE, v. 213, n. 3, p. 235-244, . (12/00786-1, 12/20622-3, 11/21395-8)
SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; MIGUITA, LUCYENE; DE ANDRADE, NATHALIA PAIVA; HEGUEDUSCH, DANIELE; RODINI, CAMILA OLIVEIRA; MOYSES, RAQUEL AJUB; TOPORCOV, TATIANA NATASHA; GAMA, RICARDO RIBEIRO; TAJARA, ELOIZA ELENA; NUNES, FABIO DAUMAS. GLI3 knockdown decreases stemness, cell proliferation and invasion in oral squamous cell carcinoma. International Journal of Oncology, v. 53, n. 6, p. 2458-2472, . (12/00786-1, 11/21395-8)
SEDASSARI, BRUNO TAVARES; SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; MARIANO, FERNANDA VIVIANE; ALTEMANI, ALBINA; NUNES, FABIO DAUMAS; SOUSA, SUZANA. The Stem Cell Marker Bmi-1 Is Sensitive in Identifying Early Lesions of Carcinoma ex Pleomorphic Adenoma. MEDICINE, v. 94, n. 27, . (12/00786-1)
SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; SEDASSARI, BRUNO TAVARES; ESTEVES, CARINA MAGALHAES; DE ANDRADE, NATHALIA PAIVA; ALTEMANI, ALBINA; ORSINI MACHADO DE SOUSA, SUZANA CANTANHEDE; NUNES, FABIO DAUMAS. Embryonic stem cells markers Oct4 and Nanog correlate with perineural invasion in human salivary gland mucoepidermoid carcinoma. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 46, n. 2, p. 112-120, . (12/00786-1)

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