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Ultrastructure and morphometry of the aortic depressor nerve in experimental diabetes: effects of insulin treatment

Grant number: 12/00231-0
Support Opportunities:Regular Research Grants
Duration: June 01, 2012 - May 31, 2014
Field of knowledge:Biological Sciences - Morphology - Anatomy
Principal Investigator:Valéria Paula Sassoli Fazan
Grantee:Valéria Paula Sassoli Fazan
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Few reports deal with the diabetes effects on unmyelinated fiber morphology and how insulin treatment affects these fibers. The aortic depressor nerve (ADN) is the afferent arm of the baroreflex, and its myelinated fibers were already investigated in experimental diabetes (Fazan et al., 2006) while the unmyelinated fibers were never studied in this experimental model. We will evaluate the ultrastructural morphometric alterations in the ADN of rats, due to acute and chronic experimental diabetes and will study the effects of conventional insulin treatment in these alterations. Wistar rats will receive a single intravenous injection of streptozotocin (40mg/Kg) 15 days (acute) or 12 weeks (chronic) before the experiments. Control animals will receive vehicle. Insulin treated animals will receive a single subcutaneous injection of insulin on a daily basis. Under pentobarbital anesthesia the ADNs will be isolated and proximal and distal segments prepared for transmission electron microscopy as reported previously (Fazan et al., 1997; Fazan et al., 2001). Morphometry will be carried out with the aid of computer software and will take into consideration the number of the unmyelinated fibers and their density, as well as the area and diameter of these fibers. Endoneural blood vessels and myelinated fibers will also be investigated for ultrastructural alterations. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DO CARMO, ELISABETE DE CASSIA; SANADA, LUCIANA SAYURI; BERTO MACHADO, NATHALIA LEILANE; SASSOLI FAZAN, VALERIA PAULA. Does Pain in the Neonatal Period Influence Motor and Sensory Functions in a Similar Way for Males and Females During Post-Natal Development in Rats?. PAIN MEDICINE, v. 17, n. 8, p. 1520-1529, . (09/07265-4, 13/01111-0, 10/04116-5, 12/00231-0)

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