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Leptospira and equine herpesvirus equino-1 as casual agents of equine abortion

Abstract

The seroepidemiological surveys indicate that Leptospira and equine herpesvirus are spread in the horse herds of Brazil. The economic importance of these infections is due to the losses on spending with laboratorial diagnosis and treatment, abortion and its complications in reproductive efficiency, decreased performance in competition and work, besides the fact that leptospirosis is a zoonosis. The Laboratory of Anatomopathology of the Biological Institute made in 2009 a study of 25 equine aborted fetuses using histopathological techniques as Hematoxylin / eosin (HE) and Warthin-Starry staining in fragments of liver and kidneys for Leptospira spp research, and in the years 2010 to 2011 a study of 42 equine aborted fetuses / stillborn using the technique of direct immunofluorescence and HE. In these studies, the results were negative to both agents, although there were observed histological lesions suggestive of infectious abortion by HE staining. The majority of epidemiological studies of leptospirosis and equine abortion virus are made by serological analysis, isolation of the agent, and biomolecular studies are used as direct diagnosis of the agent. Once the immunohistochemistry (IHC) gives three important information: the type and location of the lesions and the presence of antigens in there, it´s proposed by the present of this project the use of IHC in the research of leptospira and equine herpesvirus in equine aborted fetuses / stillbirths referred to the Center for Research and Development of Animal Sanity of the Biological Institute for the differential diagnosis of equine abortion. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALINE APARECIDA DA SILVA; ELENICE MARIA SEQUETIN CUNHA; MARIA DO CARMO CUSTÓDIO DE SOUZA HUNOLD LARA; ELIANA MONTEFORTE CASSARO VILLALOBOS; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR; ENIO MORI; CAROLINA NATALIA ZANUZZI; CECÍLIA MÔNICA GALOSI; CLAUDIA DEL FAVA. Baixa ocorrência de herpes-vírus equino 1 (HVE-1) como causa de abortamento e mortalidade perinatal no Brasil. Arq. Inst. Biol., v. 85, . (12/00277-0)
ALINE APARECIDA DA SILVA; ELIANA MONTEFORTE CASSARO VILLALOBOS; ELENICE MARIA SEQUETIN CUNHA; MARIA DO CARMO CUSTÓDIO DE SOUZA HUNOLD LARA; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR; ROSA MARIA PIATTI; VANESSA CASTRO; ELIANA SCARCELLI PINHEIRO; ALINE FEOLA DE CARVALHO; CLAUDIA DEL FAVA. Causas de abortamento, natimortalidade e mortalidade em equinos no Brasil. Arq. Inst. Biol., v. 87, . (12/00277-0, 09/13864-8)

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