Advanced search
Start date

Insect digestion: a molecular, cellular, physiological and evolutionary approach


The objective of this project is to describe the molecular events underlying midgut physiological phenomena of insect types pertaining to different orders. The aim of these studies is to find out general principles from which hypotheses can be advanced in relation to the digestive processes of insects not previously studied. Such studies are necessary for developing new insect control procedures. The project is divided into 4 parts: (a) Insect-purified or recombinant digestive enzymes in a search for peculiarities that set them apart from vertebrate enzymes and that led insects to overcome plant defense; (b) Midgut molecular physiology, which models will be based on cDNA deep sequencing associated with data on digestive enzymes, buffering systems, and transporters, etc .. The models will be tested by protein silencing with the aid of RNAi; (c) Digestive secretory mechanisms that are unique to insects. Secretory models will be advanced based on transcriptomes prepared from chosen midgut regions and the models will be tested as in (b); (d) Evolution of insect digestive systems. The present proposal will be improved by studying insect groups not considered before, such as leafhoppers, thrips, and stick insects and by "phylotranscriptomics", the comparative analysis of whole or part of the midgut transcriptomes (obtained by pyrosequencing) we have available or intend to prepare. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FUZITA, FELIPE JUN; CHANDLER, KEVIN BROWN; HASERICK, JOHN R.; TERRA, WALTER R.; FERREIRA, CLELIA; COSTELLO, CATHERINE E.. N-glycosylation in Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 246-24, . (11/51685-8, 16/09511-6)
DAMASCENO, TICIANE F.; DIAS, RENATA O.; DE OLIVEIRA, JULIANA R.; SALINAS, ROBERTO K.; JULIANO, MARIA A.; FERREIRA, CLELIA; TERRA, WALTER R.. Active subsite properties, subsite residues and targeting to lysosomes or midgut lumen of cathepsins L from the beetle Tenebrio molitor. Insect Biochemistry and Molecular Biology, v. 89, p. 17-30, . (15/19541-7, 11/51685-8, 16/07490-1)

Please report errors in scientific publications list by writing to: