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Subgingival microbiota diversity and serum and gingival fluid cytokines profile related to glycemic control in subjects with Type II Diabetes

Grant number: 11/18618-5
Support Opportunities:Regular Research Grants
Duration: March 01, 2012 - August 31, 2014
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Marcia Pinto Alves Mayer
Grantee:Marcia Pinto Alves Mayer
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers:Priscila Larcher Longo


There is a bidirectional relationship between periodontitis and diabetes mellitus type II, where the treatment of periodontal infection can be considered part of the treatment of diabetes, while glycemic control is a prerequisite for successful periodontal therapy. The inflammatory markers and the subgingival microbiota of periodontitis cases associated with diabetes with blood sugar imbalance of cases of chronic periodontitis were still not differentiated from other diabetics and nondiabetics periodontitis, although they show distinct severity and prognosis. This study aims to test the hypothesis that the subgingival microbiota of diabetic patients with glucose imbalance differs from the microbiota of diabetic patients with glucose balance and non-diabetic controls, reflecting and / or being reflected by the exacerbated inflammatory condition in cases of failure of glucose balance. 30 patients with periodontitis will be selected 10 without diabetes, 10 diabetics with glycemic control and 10 diabetics with poor glycemic control. Patients will undergo medical examinations, blood sampling, clinical periodontal examination and collection of samples of subgingival and gingival fluid. The diversity of the subgingival microbiota will be evaluated by 16SrRNA genomic libraries. Biofilm samples from shallow and deep periodontal sites will be evaluated for levels of recognized periodontal pathogens and selected organisms based on diversity data by RNA-oligonucleotide quantification technique (ROQT). Gingival fluid and serum samples will be analyzed for cytokines and bone resorption mediators. The data will provide evidences of specific markers for periodontitis in diabetic patients and their relation to glucose balance to be tested in further longitudinal studies. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LONGO, PRISCILA L.; DABDOUB, SHAREEF; KUMAR, PURNIMA; ARTESE, HILANA P. C.; DIB, SERGIO A.; ROMITO, GIUSEPPE A.; ALVES MAYER, MARCIA PINTO. Glycaemic status affects the subgingival microbiome of diabetic patients. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 45, n. 8, p. 932-940, . (13/20537-9, 11/10057-4, 11/18618-5)
PRISCILA LARCHER LONGO; HILANA PAULA CARILLO ARTESE; MARIANADE SOUSA RABELO; DIONE KAWAMOTO; ADRIANA MOURA FOZ; GIUSEPPE ALEXANDRE ROMITO; SÉRGIO ATALA DIB; MARCIA PINTO ALVES MAYER. Serum leveis of inflammatory markers in type 2 diabetes patients with chronic periodontitis. Journal of Applied Oral Science, v. 22, n. 2, p. 103-108, . (11/18618-5)
LONGO, PRISCILA LARCHER; CARILLO ARTESE, HILANA PAULA; RABELO, MARIANA DE SOUSA; KAWAMOTO, DIONE; FOZ, ADRIANA MOURA; ROMITO, GIUSEPPE ALEXANDRE; DIB, SERGIO ATALA; ALVES MAYER, MARCIA PINTO. Serum levels of inflammatory markers in type 2 diabetes patients with chronic periodontitis. Journal of Applied Oral Science, v. 22, n. 2, p. 103-108, . (11/18618-5)

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