Research Grants 11/17665-0 - Transplante de córnea, Células-tronco mesenquimais - BV FAPESP
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Corneal reparation (Part I and Part II)

Abstract

Part I: Mesenchymal stem cells are considered adult pluripotent cells that, after isolation by cell culture and expansion, they are able to differentiate into different tissues. With the bioengineered tissue, applied to ophthalmology, have been sought membranes and other materials that can be integrated into the cornea facilitating the repair without loss of corneal transparency. Recent research has demonstrated the capability of the omentum in expressing such cells, arranged in a thin membrane fenestrated richly vascularized, keeping growth factors, angiogenic, neurotransmitters and inflammatory mediators. The amniotic membrane can be used as a substrate for growing cells, whose sources may be in limbo, the oral mucosa, conjunctiva and amniotic membrane itself. The efficacy of amniotic membrane in corneal corroborate the repair has been confirmed. With the project will be to compare pa lamellar keratoplasty by omentum graft preserved in glycerin at 98%, enriched or not with mesenchymal stem cells, obtained from the omentum of rabbits with amniotic membrane enriched with mesenchymal stem cells from the same omentum. Proceed will be the cell culture. The omentum and the amniotic membrane, enriched or not. The results will be evaluated clinically and laboratory.Part II: Deep corneal stromal diseases such as corneal dystrophy, keratoconus, corneal abscesses, keratomalacia, and iris prolapse are relatively common in humans and animals. Corneal transplantation may be useful to manage these abnormalities with penetrating keratoplasty (PK) one of the most widely used techniques for this purpose. This study will evaluate the endothelial cellularity by non-contact specular microscopy, histology and scanning electron microscopy, following penetrating keratoplasty in rabbits. For the study, 18 adult healthy rabbits, males and females, with weight about 3.0 kg will be used. Animals will be divided into 6 groups (G1 - G6) composed of 3 animals each. Non-contact specular microscopy will be carried out prior to penetrating keratoplasty in all animals. Following surgery, the animals will be evaluated daily for 30 days and every 3 days after that period for up to 60 days. Euthanasia will be performed on days 3 (G1), 15 (G2), 21 (G3), 30 (G4), 45 (G5) and 60 (G6) after surgeries. Following euthanasia, corneas will be collected and submitted to histology and scanning electron microscopy. Data will be evaluated using logistic regression and SAS software - Statistical Analysis System. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SÉFORA VIEIRA DA SILVA GOUVÊA DE BARROS; MARCELA ALDROVANI; LUCIANA CENÇO CORREA DE LACERDA; MÔNICA HORR; FÁBIO ANDRADE MARINHO; TIAGO BARBALHO LIMA; CAMILA PINHO BALTHAZAR DA SILVEIRA; FÁBIO LUIZ DA CUNHA BRITO; VINÍCIUS BASSANEZE; MAYRA CUNHA FLECHER; et al. Ceratoplastia lamelar em coelhos usando enxerto de omento alógeno livre e células mesenquimais omentais associadas com membrana amniótica canina. Ciência Rural, v. 46, n. 10, p. 1838-1845, . (11/17665-0, 12/17308-5)
DA SILVA GOUVEA DE BARROS, SEFORA VIEIRA; ALDROVANI, MARCELA; CORREA DE LACERDA, LUCIANA CENCO; HORR, MONICA; MARINHO, FABIO ANDRADE; LIMA, TIAGO BARBALHO; BALTHAZAR DA SILVEIRA, CAMILA PINHO; DA CUNHA BRITO, FABIO LUIZ; BASSANEZE, VINICIUS; FLECHER, MAYRA CUNHA; et al. Lamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum- derived mesenchymal cells associated with the canine amniotic membrane. Ciência Rural, v. 46, n. 10, p. 1838-1845, . (11/17665-0, 12/17308-5)

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