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Effects of low level laser on regulatory myogenic factors expression and matrix metalloprotease 2 activity during skeletal muscle repair following cryolesion in rats

Abstract

Low level laser therapy (LLLT) is widely used in order to promote faster and better quality repair muscle after different types of injury, however, many studies show controversies in the literature with regard to parameters. The aim of the present study is to determine the effect of LLLT on the expression of myogenic regulatory factors (MyoD, Myf5, myogenin and MRF4) and on matrix metalloprotease 2 (MMP2) activity in the tibialis anterior (TA) muscle of rats following cryoinjury. Methods: Adult male Wistar rats (n=65) will be randomly divided into five groups: (1) control (no lesion, untreated,; (2) Sham group (without lesion and muscle will be only exposed); (3) cryoinjury group euthanized after 1, 3 and 7 days; (4) cryoinjury LLLT group euthanized after 1, 3 and 7 days; (5) without lesion euthanized after 1, 3 and 7 days. Laser irradiation will be performed three times a week on the injured region using the AsGaAl laser (780 nm; beam spot of 0.04 cm2, output power of 40 mW, energy density of 10 J/cm2, 10-second exposure time). The muscles will be removed and to isolate the total RNA using TRIzol reagent and to obtain cDNA synthesis. Real-time polymerase chain reactions will be performed using primers for MyoD, Myf5, myogenin and MRF4 and GAPDH was used to normalize the data. In addition, MMP2 activity will analyze by zimography and its presence will be confirmed by Western Blotting. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES, A. N.; FERNANDES, K. P. S.; MELO, C. A. V.; YAMAGUCHI, R. Y.; FRANCA, C. M.; TEIXEIRA, D. F.; BUSSADORI, S. K.; NUNES, F. D.; MESQUITA-FERRARI, R. A.. Modulating effect of low level-laser therapy on fibrosis in the repair process of the tibialis anterior muscle in rats. Lasers in Medical Science, v. 29, n. 2, SI, p. 813-821, . (11/04452-8, 11/17638-2)
ALVES, A. N.; FERNANDES, K. P. S.; MELO, C. A. V.; YAMAGUCHI, R. Y.; FRANCA, C. M.; TEIXEIRA, D. F.; BUSSADORI, S. K.; NUNES, F. D.; MESQUITA-FERRARI, R. A.. Modulating effect of low level-laser therapy on fibrosis in the repair process of the tibialis anterior muscle in rats. Lasers in Medical Science, v. 29, n. 2, p. 9-pg., . (11/04452-8, 11/17638-2)
ALVES, AGNELO NEVES; RIBEIRO, BEATRIZ GUIMARES; SANTOS FERNANDES, KRISTIANNE PORTA; COSTA SOUZA, NADHIA HELENA; ROCHA, LILIA ALVES; NUNES, FABIO DAUMAS; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. Comparative effects of low-level laser therapy pre- and post-injury on mRNA expression of MyoD, myogenin, and IL-6 during the skeletal muscle repair. Lasers in Medical Science, v. 31, n. 4, p. 679-685, . (14/12381-1, 13/21540-3, 12/11461-6, 11/04452-8, 11/17638-2)
ALVES, AGNELO NEVES; SANTOS FERNANDES, KRISTIANNE PORTA; DEANA, ALESSANDRO MELO; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. Effects of Low-Level Laser Therapy on Skeletal Muscle Repair A Systematic Review. AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION, v. 93, n. 12, p. 1073-1085, . (11/17638-2)
FRANCA, CRISTIANE MIRANDA; SANTANA, CRISTIANO DE LOURA; TAKAHASHI, CAMILA BORIN; ALVES, AGNELO NEVES; DE SOUZA MERNICK, ANA PAULA; SANTOS FERNANDES, KRISTIANNE PORTA; TEIXEIRA DA SILVA, DANIELA DE FATIMA; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. Effect of laser therapy on skeletal muscle repair process in diabetic rats. Lasers in Medical Science, v. 28, n. 5, p. 1331-1338, . (11/04452-8, 11/20867-3, 11/17638-2)
SANTOS FERNANDES, KRISTIANNE PORTA; ALVES, AGNELO NEVES; NUNES, FABIO DAUMAS; COSTA SOUZA, NADHIA HELENA; SILVA, JR., JOSE ANTONIO; BUSSADORI, SANDRA KALIL; MESQUITA FERRARI, RAQUEL AGNELLI. Effect of photobiomodulation on expression of IL-1 beta in skeletal muscle following acute injury. Lasers in Medical Science, v. 28, n. 3, p. 1043-1046, . (11/04452-8, 11/17638-2)

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