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Evaluation of AFB1-Lisin and AFB1-N7-guanin aducts as biomarkers of human and animal exposure to aflatoxins

Grant number: 10/20895-4
Support Opportunities:Research Projects - Thematic Grants
Duration: November 01, 2011 - April 30, 2016
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Carlos Augusto Fernandes de Oliveira
Grantee:Carlos Augusto Fernandes de Oliveira
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Pesquisadores principais:
Leandra Náira Zambelli Ramalho
Associated researchers:Alessandra Vincenzi Jager ; Andrezza Maria Fernandes ; Fernando Gustavo Tonin ; Fernando Silva Ramalho ; Lúcio Francelino Araújo ; Ricardo de Albuquerque
Associated scholarship(s):15/06049-7 - Aflatoxin B1 biomarkers determination and applicability for efficiency evaluation of adsorbents in swine, BP.IC
13/23210-0 - Evaluation of AFB1-lysine synthesis and stability procedures for analytical determinations, BP.IC
13/04627-8 - Correlation between dietary intake of aflatoxin B1, hepatic expression of the genes and proteins related to hepatocarcinogenesis, and concentration of AFB1-adducts in serum, urine and liver of Wistar rats, BP.DR
+ associated scholarships 12/12705-6 - Determination of aflatoxin B1 biomarkers and its applicability for evaluation of adsorbents efficiency in pigs, BP.DD
12/07726-4 - Determination of Aflatoxin B1 biomarkers and its aplicability on efficience avaliation of adsorbents in broiler chickens., BP.DR
10/08746-3 - Assessment of human exposure to aflatoxins in foods by evaluating some biomarkers in residents of rural areas in Pirassununga/SP, BP.PD - associated scholarships


Aflatoxins are carcinogenic compounds produced by Aspergillus species, which can contaminate foods before and after processing, leading to human health risks and losses in the animal production. The exposure to aflatoxin B1 (AFB1), the metabolite with higher toxicity produced by the fungus, occurs predominantly through the ingestion of contaminated food, mainly corn, peanuts and by-products, which have been frequently reported as AFB1-contaminated products in Brazil. AFB1 is primarily bio-transformed by microssomal enzymes in the liver, originating AFB1-N7-guanine and AFB1-lysin adducts. This thematic project is constituted by 6 sub-projects, which have independent and complementary objectives for generation of knowledge on AFB1 adducts and their application as biomarkers of exposure to aflatoxins in animals and humans. The involvement of AFB1 and its adducts with human hepatocellular carcinoma (HCC) will also be investigated. The themes of the sub-projects are: Synthesis of AFB1-lysine and AFB1-N7-guanine analytical standards (sub-project I), for using in the sub-projects; Establishment of dose-response relationships between the concentration of AFB1-aducts in biological materials and molecular changes in experimental model of HCC (sub-project IV); Establishment of dose-response relationships between the intake AFB1 and concentration of AFB1-adducts in biological materials and molecular changes of CHC in humans (sub-projects II and III); Application of analysis of AFB1-adducts in biological materials, as biomarkers for evaluation of adsorbents in trials with broilers and pigs (sub-projects V and VI). (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRAZ, MARIANA M.; ROQUE-BARREIRA, MARIA CRISTINA; RAMALHO, FERNANDO S.; OLIVEIRA, CARLOS A.; AUGUSTO, MARLEI J.; RAMALHO, LEANDRA N. Z.. Inhibition of Hepatocarcinogenesis by ArtinM via Anti-proliferative and Pro-apoptotic Mechanisms. IN VIVO, v. 30, n. 6, p. 845-852, . (10/20895-4)
RAMALHO, F. N.; SANCHES, S. C.; FOSS, M. C.; AUGUSTO, M. J.; SILVA, D. M.; OLIVEIRA, A. M.; RAMALHO, L. N.. Aliskiren effect on non-alcoholic steatohepatitis in metabolic syndrome. DIABETOLOGY & METABOLIC SYNDROME, v. 9, . (10/20895-4)
DI GREGORIO, MAYRA CARRARO; JAGER, ALESSANDRA VINCENZI; MAGGIO CASTRO SOUTO, POLLYANA CRISTINA; COSTA, ALINE ALVES; ROTTINGHAUS, GEORGE EDWIN; PASSARELLI, DANIELLE; LEMOS BUDINO, FABIO ENRIQUE; CORASSIN, CARLOS HUMBERTO; FERNANDES OLIVEIRA, CARLOS AUGUSTO. Determination of serum aflatoxin B-1-lysine to evaluate the efficacy of an aflatoxin-adsorbing feed additive in pigs fed an aflatoxin B-1-contaminated diet. MYCOTOXIN RESEARCH, v. 33, n. 2, p. 93-102, . (12/12705-6, 10/20895-4)
JAGER, ALESSANDRA V.; TONIN, FERNANDO G.; BAPTISTA, GABRIELA Z.; SOUTO, POLLYANA C. M. C.; OLIVEIRA, CARLOS A. F.. Assessment of aflatoxin exposure using serum and urinary biomarkers in Sao Paulo, Brazil: A pilot study. INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, v. 219, n. 3, p. 294-300, . (10/20895-4)
DI GREGORIO, MAYRA C.; JAGER, ALESSANDRA V.; COSTA, ALINE A.; BORDIN, KELIANI; ROTTINHGHAUS, GEORGE E.; PETTA, TANIA; SOUTO, POLLYANA C. M. C.; BUDINO, FABIO E. L.; OLIVEIRA, CARLOS A. F.. Determination of Aflatoxin B-1-Lysine in Pig Serum and Plasma by Liquid Chromatography-Tandem Mass Spectrometry. JOURNAL OF ANALYTICAL TOXICOLOGY, v. 41, n. 3, p. 236-241, . (12/12705-6, 10/20895-4)
APOLINARIO, LETICIA A.; RAMALHO, LEANDRA N. Z.; MOOSAVI, MOTAHAREH HASHEMI; JAGER, V, ALESSANDRA; AUGUSTO, MARLEI J.; TROTTA, MAURICIO R.; PETTA, TANIA; KHANEGHAH, AMIN MOUSAVI; OLIVEIRA, CARLOS A. F.; RAMALHO, FERNANDO S.. Oncogenic and tumor suppressor pathways in subchronic aflatoxicosis in rats: Association with serum and urinary aflatoxin exposure biomarkers. Food and Chemical Toxicology, v. 153, . (10/20895-4)
RAMALHO, LEANDRA N. Z.; PORTA, LIVIA D.; ROSIM, ROICE E.; PETTA, TANIA; AUGUSTO, MARLEI J.; SILVA, DEISY M.; RAMALHO, FERNANDO S.; OLIVEIRA, CARLOS A. F.. Aflatoxin B-1 residues in human livers and their relationship with markers of hepatic carcinogenesis in Sao Paulo, Brazil. TOXICOLOGY REPORTS, v. 5, p. 777-784, . (10/20895-4)

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