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Green coffee oil extraction by supercritical and pressurized fluid process, and comparison with the composition of coffee oil obtained by pressing for the small producers of the State of São Paulo - concentration analysis of bioactive diterpenes


In 2008, Brazil produced approximately 2.8 million tons of coffee, accounting for 34.3% of world production (ABIC, 2010). In the ranking of world trade in dollars, the coffee industry is the second largest following only petrol oil. These data are indicative that the coffee is still a significant agricultural product of commercial importance to Brazil. However, excess world supply beyond demand has reduced its value in the last three decades and this comportment encourages the development of coffee products with higher added value in order to reduce this trend.This research project, with the support of small oil producers of green coffee from Brazil, aims to study the composition of coffee oil obtained by them, emphasizing the phenolic compounds and diterpenes cafestol and kahweol. These components are relevant to the cosmetic and pharmaceuticals industries. The concentration of these and other minor components in coffee oil is that classify its value.Although knowledge on the applicability of green coffee oil products for health, there is no literature on technical/scientific a standardized description of the composition of Brazilian green coffee oil obtained after the processing of grain. There are many works that present the characterization of minor components present in grains, but this composition may not be consistent with the oil obtained by pressing, since the extractions in the laboratory scale have characteristics very different from those applied in processing. Thus, this research aims to demonstrate possible changes in the conventional extraction process and to complement this study with the use of new methods that can serve as subsidies to encourage innovation of rudimentary processes.Besides establishing the composition of Standard Oil of Brazilian green coffee, this project aims to employ new extractions methods and using them to demonstrate that this oil can be enriched. Longer period, we want to encourage technological development by studying the possibility of using these news extraction processes as large-scale.For achieved the proposed objectives, the methods of pressurized fluid extraction (PFE) and supercritical fluid extraction (SFE) will be employed and the composition, obtained by the use of analytical methods of separation (Thin Layer Chromatography-TLC and Gas Chromatography/Mass Spectrometry-GC/MS) are the tools used to demonstrate that the use of these processes could enrich the oil of green coffee in the minor components, emphasizing the diterpenes cafestol and kahweol. (AU)

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Scientific publications (12)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BITENCOURT, RAPHAELA G.; PALMA, ANDRE M.; COUTINHO, JOAO A. P.; CABRAL, FERNANDO A.; MEIRELLES, ANTONIO J. A.. Prediction of solid solute solubility in supercritical CO2 with cosolvents using the CPA EoS. Fluid Phase Equilibria, v. 482, p. 1-10, . (14/21252-0, 10/16665-3)
SANTOS, DEBORA NASCIMENTOE; DE SOUZA, LARISSA LIMA; FERNANDES DE OLIVEIRA, CARLOS AUGUSTO; SILVA, EDSON ROBERTO; DE OLIVEIRA, ALESSANDRA LOPES. Arginase inhibition, antibacterial and antioxidant activities of Pitanga seed (Eugenia uniflora L.) extracts from sustainable technologies of high pressure extraction. FOOD BIOSCIENCE, v. 12, p. 93-99, . (08/00148-0, 10/16665-3)
MACHADO, L. C.; PELEGATI, V. B.; OLIVEIRA, A. L.. Study of simple microparticles formation of limonene in modified starch using PGSS - Particles from gas-saturated suspensions. JOURNAL OF SUPERCRITICAL FLUIDS, v. 107, p. 260-269, . (12/01221-8, 10/16665-3, 08/00148-0, 12/06981-0)
APARECIDA DE OLIVEIRA, PAOLA MARESSA; DE ALMEIDA, RAFAEL HENRIQUE; DE OLIVEIRA, NAILA ALBERTINA; BOSTYN, STEPHANE; GONCALVES, CINTIA BERNARDO; DE OLIVEIRA, ALESSANDRA LOPES. Enrichment of diterpenes in green coffee oil using supercritical fluid extraction - Characterization and comparison with green coffee oil from pressing. JOURNAL OF SUPERCRITICAL FLUIDS, v. 95, p. 137-145, . (10/16665-3)
PUTRINO, FERNANDO MARQUES; TEDESCO, MARCELA; BODINI, RENATA BARBOSA; DE OLIVEIRA, ALESSANDRA LOPES. Study of supercritical carbon dioxide pretreatment processes on green coconut fiber to enhance enzymatic hydrolysis of cellulose. Bioresource Technology, v. 309, . (10/16665-3)
VILLEGAS, MARIA EUGENIA; AREDO, VICTOR; EGG ASEVEDO, KAYQUE JULIO; LOURENCO, RODRIGO VINICIUS; BAZITO, REINALDO CAMINO; OLIVEIRA, ALESSANDRA LOPES. Commercial Starch Behavior When Impregnated with Food Additives by Moderate Temperature Supercritical CO(2)Processing. STARCH-STÄRKE, v. 72, n. 11-12, . (08/00148-0, 10/16665-3, 12/06981-0)
BITENCOURT, RAPHAELA G.; PALMA, ANDRE M.; COUTINHO, JOAO A. P.; CABRAL, FERNANDO A.; MEIRELLES, ANTONIO J. A.. Solubility of caffeic acid in CO2 + ethanol: Experimental and predicted data using Cubic Plus Association Equation of State. JOURNAL OF SUPERCRITICAL FLUIDS, v. 138, p. 238-246, . (10/16665-3, 14/21252-0)
BELANDRIA, VERONICA; APARECIDA DE OLIVEIRA, PAOLA MARESSA; CHARTIER, AGNES; RABI, JOSE A.; DE OLIVEIRA, ALESSANDRA LOPES; BOSTYN, STEPHANE. Pressurized-fluid extraction of cafestol and kahweol diterpenes from green coffee. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 37, n. A, p. 145-152, . (10/16665-3)
COLIVET, J.; OLIVEIRA, A. L.; CARVALHO, R. A.. Influence of the bed height on the kinetics of watermelon seed oil extraction with pressurized ethanol. Separation and Purification Technology, v. 169, p. 187-195, . (10/16665-3)
BITENCOURT, RAPHAELA G.; FERREIRA, NILSON J.; OLIVEIRA, ALESSANDRA L.; CABRAL, FERNANDO A.; MEIRELLES, ANTONIO J. A.. High pressure phase equilibrium of the crude green coffee oil - CO2 - ethanol system and the oil bioactive compounds. JOURNAL OF SUPERCRITICAL FLUIDS, v. 133, n. 1, p. 49-57, . (10/16665-3, 14/21252-0)
COMELIO-SANTIAGO, HEBER P.; GONCALVES, CINTIA BERNARDO; DE OLIVEIRA, NAILA ALBERTINA; DE OLIVEIRA, ALESSANDRA LOPES. Supercritical CO2 extraction of oil from green coffee beans: Solubility, triacylglycerol composition, thermophysical properties and thermodynamic modelling. JOURNAL OF SUPERCRITICAL FLUIDS, v. 128, p. 386-394, . (10/16665-3)
DE OLIVEIRA, NAILA ALBERTINA; CORNELIO-SANTIAGO, HEBER P.; FUKUMASU, HEIDGE; DE OLIVEIRA, ALESSANDRA LOPES. Green coffee extracts rich in diterpenes - Process optimization of pressurized liquid extraction using ethanol as solvent. Journal of Food Engineering, v. 224, p. 148-155, . (13/03371-0, 10/16665-3)

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