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Occurrence of the infection by Cryptosporidium spp. in water buffaloes, goats and sheeps


Cryptosporidiosis has a worldwide distribution and can affect animals and humans, especially young and immunosuppressed. This research project aims to assess the occurrence of the infection by Cryptosporidium spp. in young buffaloes, goats and sheep. For this, will collected fecal samples from a total of 200 buffalo calves, 260 kid goats and 200 lambs. To verify the elimination of oocysts, the feces will be examined with optical microscopy after stained by Kinyoun modified. For detection of the parasite antigen, will employed the kit ELISA Cryptosporidium Test of TechLab®. The positive samples by microscopy or ELISA test will be molecularly characterized by the technique Nested-PCR. Statistical analysis will consist of the Chi-Square test, using the SAS statistical software with the 5% level of significance, will be checked the association between the parasite with sex, race and age of animals examined. Will compared to the efficacy the Kinyoun and ELISA tests by diagnostic the Cryptosporidium spp. in the samples analyzed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
INACIO, SANDRA VALERIA; WIDMER, GIOVANNI; LEMOS DE BRITO, ROBERTA LOMONTE; MIRANDA OLIVEIRA, BRUNO CESAR; NAKAMURA, ALEX AKIRA; NETO, LUIZ DA SILVEIRA; BALIZARDO CARVALHO, JOAO GABRIEL; MEIRELES, MARCELO VASCONCELOS; SARAIVA BRESCIANI, KATIA DENISE. First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil. Veterinary Parasitology, v. 233, p. 48-51, JAN 15 2017. Web of Science Citations: 10.
AQUINO, MONALLY C. C.; WIDMER, GIOVANNI; ZUCATTO, ANAIZA S.; VIOL, MILENA A.; INACIO, SANDRA V.; NAKAMURA, ALEX A.; COELHO, WILLIAN M. D.; PERRI, SILVIA H. V.; MEIRELES, MARCELO V.; BRESCIANI, KATIA D. S. First Molecular Characterization of Cryptosporidium spp. Infecting Buffalo Calves in Brazil. Journal of Eukaryotic Microbiology, v. 62, n. 5, p. 657-661, SEP-OCT 2015. Web of Science Citations: 3.

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