Genetic mapping and identification of genetic and functional molecular markers associated with agronomic characteristics of interest in sugarcane

Abstract

In Brazil, the sugarcane agribusiness turns over around R$ 36 billion each year. The mapping of QTLs relating to sugar levels and the components of production (number of profiles, diameter and height of stalks) is of great importance to sugarcane improvement. Sucest has enabled the development of functional markers for the genetic mapping. Such markers are ideal for assisted selection, given that these markers may be responsible for the characteristic in question. The present project aims to initiate a program of genetic mapping for the Cana IAC program. Molecular markers of the AFLP type, genomic and functional microsatellites (EST-SSRs) with homology to genes of interest will be used for the construction of a genetic map starting from a biparental crossing, between elite materials of the program. It is intended to detect and map QTLs associated with sugar levels and to the components of production and to verify the association of RFLPsPCRs markers developed from ESTs corresponding to genes expressed during the interaction with Puccinia melanocephala, with resistance to rust. The construction of this genetic map will assist in the validation of the genomic and functional markers associated with agronomic characteristics of interest identified in other sugarcane maps, giving support in the application of these markers for assisted selection. (AU)