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Biodiversity of freshwater microalgae: germplasm bank and obtaining of molecular markers of cryopreserved species

Grant number: 11/50054-4
Support Opportunities:BIOTA-FAPESP Program - Thematic Grants
Duration: August 01, 2011 - July 31, 2017
Field of knowledge:Biological Sciences - Botany - Applied Botany
Principal Investigator:Armando Augusto Henriques Vieira
Grantee:Armando Augusto Henriques Vieira
Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Pesquisadores principais:
Célia Leite SantAnna
Associated grant(s):14/13345-9 - 17th Workshop of the International Association of Phytoplankton Taxonomy and Ecology (IAP), AR.EXT
Associated scholarship(s):16/08546-0 - Methodology development of Fourier transform infrared spectroscopy (FTIR) for microalgae discrimination, BP.IC
16/07089-5 - Biogeography of Selenastraceae (Chlorophyceae, Sphaeropleales): cosmopolitanism and distribution pattern in the light of molecular systematics, BP.PD
15/19263-7 - New primers tests for the rbcL gene amplification in Chlorophyta, BP.IC
+ associated scholarships 13/18083-0 - Selenastraceae (Chlorococcales) family: evaluation of molecular marker potencies for DNA barcoding and for phylogeny of the genera Monoraphidium and Ankistrodesmus, BP.PD
13/03979-8 - Profile of fatty acids in freshwater microalgae species, family Selenastraceae, Chlorophyta, as chemotaxonomic characteristic and prospecting applicability, BP.DD
12/19520-1 - Biodiversity of Selenastraceae (freshwater Chlorococcaceae microalgae): morphological characteristics and sequencing of 18S rDNA as a traditional taxonomic basis for obtaining the DNA barcode, BP.DD
11/18327-0 - Criopreservation of germplasm of freshwater microalgae: methodology development, BP.DR
12/00221-4 - Extracellular polysaccharides of microalgae: chemotaxonomy and prospect interactions in nanostructured films, BP.DR - associated scholarships


The purpose of the project is to begin a germplasm bank, cryopreserved in liquid nitrogen, of freshwater microalgae (phytoplanktonic, but also thycoplanktonic, benthic and sub-aerial which may be cultivated and frozen), in order to maintain the biodiversity of these organisms, along with a database of information on the species. The first step to achieve this intent is to isolate clones throughout the UGRHI from the State of Sao Paulo, including previously demarcated stations by the Biota Program, cultivate them and insert them in the collection of metabolically active culture of the Collection of Freshwater Microalgae Cultures of UFSCar. Immediately after isolation, we will develop freezing protocols aiming the progressive transfer of such cultures to the frozen "bank" (cryopreservation).The studies of DNA barcode will begin with Chlorophyceae, but later expanding to other groups of which DNA barcodes are already being obtained more routinely, such as diatoms and dinoflagellates. For this, the organisms will be previously identified by traditional taxonomy. Although the "DNA barcoding" is already being successfully applied for several groups of non-green algae, it does not happen with the green algae or 'higher' plants, which requires further research. We believe that this theme is also proper to begin studies for prospecting of metabolic specific molecules that may be applied as biomarkers and, therefore, interesting for the taxonomic characterization of the species to be frozen. The approach of biomarkers will start with the profiles of fatty acids and extracellular polysaccharides (monomer composition and some physical properties), which have the potential to be useful to delimit higher taxa, like orders and genera, and even species, according with literature. We will also begin, in a later stage of the project, the novel experimental approach of comparative analysis by electronic sensors of the profile of released compounds by the species as biomarkers. Besides the taxonomic utility, biomarkers yield important information on the economic point of view, which enriches the information bank of the frozen collection. The expected results are: the substantial increase in collection of metabolically active cultures, which will be gradually transferred to the collection of cryopreserved species; the availability of frozen clones along with important information about the species; the transfer of the DNA-barcode results for the BOLD, COBOL and GenBank data systems; and the creation of a database with information not only on the sequences and primers, but also about the environment, as physical and chemical characteristics of water. The list of the frozen/active collections will be inserted to the project Biota database. (AU)

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Scientific publications (10)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA, THAIS GARCIA; BOCK, CHRISTINA; SANT'ANNA, CELIA LEITE; BAGATINI, INESSA LACATIVA; WODNIOK, SABINA; HENRIQUES VIEIRA, ARMANDO AUGUSTO. Selenastraceae (Sphaeropleales, Chlorophyceae): rbcL, 18S rDNA and ITS-2 secondary structure enlightens traditional taxonomy, with description of two new genera, Messastrum gen. nov. and Curvastrum gen. nov.. Fottea, v. 17, n. 1, p. 1-19, . (12/19520-1, 13/17457-3, 11/50054-4, 13/18083-0)
MATEUS-BARROS, ERICK; DE MELO, MICHAELA L.; BAGATINI, INESSA L.; CALIMAN, ADRIANO; SARMENTO, HUGO. Local and Geographic Factors Shape the Occupancy-Frequency Distribution of Freshwater Bacteria. MICROBIAL ECOLOGY, v. 81, n. 1, . (11/50054-4, 14/14139-3)
DE MORAES, GUILHERME PAVAN; AUGUSTO HENRIQUES VIEIRA, ARMANDO. Fourier Transform Infrared with Attenuated Total Reflectance Applied to the Discrimination of Freshwater Planktonic Coccoid Green Microalgae. PLoS One, v. 9, n. 12, . (11/50054-4)
MATEUS-BARROS, ERICK; MENEGHINE, AYLAN K.; BAGATINI, INESSA LACATIVA; FERNANDES, CAMILA C.; KISHI, LUCIANO T.; VIEIRA, ARMANDO A. H.; SARMENTO, HUGO. Comparison of two DNA extraction methods widely used in aquatic microbial ecology. Journal of Microbiological Methods, v. 159, p. 12-17, . (11/50054-4, 13/18083-0, 14/14139-3, 16/07679-7)
MORI, CILENE CRISTINA; BAGATINI, INESSA LACATIVA; DA SILVA, THAIS GARCIA; PARRISH, CHRISTOPHER CHARLES; HENRIQUES VIEIRA, ARMANDO AUGUSTO. Use of fatty acids in the chemotaxonomy of the family Selenastraceae (Sphaeropleales, Chlorophyceae). Phytochemistry, v. 151, p. 9-16, . (13/03979-8, 11/50054-4, 12/19520-1, 13/18083-0)
BAGATINI, INESSA LACATIVA; EILER, ALEXANDER; BERTILSSON, STEFAN; KLAVENESS, DAG; TESSAROLLI, LETICIA PITON; HENRIQUES VIEIRA, ARMANDO AUGUSTO. Host-Specificity and Dynamics in Bacterial Communities Associated with Bloom-Forming Freshwater Phytoplankton. PLoS One, v. 9, n. 1, . (11/50054-4)
CÉLIA L. SANT'ANNA; WATSON A. GAMA; THAIS G. SILVA; ARMANDO A.H. VIEIRA. A new species of Trichodesmium (Cyanobacteria) from freshwaters, Brazil. Rodriguésia, v. 69, n. 4, p. 2019-2024, . (12/16430-1, 11/50054-4)
MELLO, RODRIGO V.; MECCHERI, FABRICIO S.; BAGATINI, INESSA L.; RODRIGUES-FILHO, EDSON; VIEIRA, ARMANDO A. H.. MALDI-TOF MS based discrimination of coccoid green microalgae (Selenastraceae, Chlorophyta). ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 28, p. 151-160, . (11/50054-4, 13/18083-0, 12/00221-4)
FREITAS, ROBERTA; VIEIRA, HELENA HENRIQUES; DE MORAES, GUILHERME PAVAN; DE MELO, MICHAELA LADEIRA; HENRIQUES VIEIRA, ARMANDO AUGUSTO; SARMENTO, HUGO. Productivity and rainfall drive bacterial metabolism in tropical cascading reservoirs. Hydrobiologia, v. 809, n. 1, p. 233-246, . (11/50054-4, 14/14139-3)

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