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Influence of green tea from the Camellia sinensis plant in the reproduction of superovulated Wistar rats and in the gene expression of vascular endothelial growth factor (VEGF) of corpus luteum

Abstract

The female reproductive organs are always growing and regressing. Because of that, they suffer continually remarkable changes in the degree of blood flow. So they are some of the few adult tissues in which the angiogenesis occurs as a normal process. Ovarian follicles and corpus luteum contain and produce angiogenic factors. The main angiogenic factors are the vascular endothelial growth factors (VEGFs). Studies show that some catechins in the Green tea inhibit the VEGFs, but there are no studies demonstrating that the chronic consumption of Green tea can interfere with reproduction. The aim of this study is to determine the influence of Green tea in ovulation rate in superovulated rats by the number of corpus luteum. Moreover, the ovarian weight will be measured and the gene expression of VEGF will be checked in the corpus luteum. For this, the rats will be divided into two groups: control group (n = 30) and group that will intake the Green tea (n = 30). The corpus luteum will be collected from 10 animals in each group at the end of each month, for three consecutive months, the number of corpora lutea and ovarian weight will be assessed. For analysis of VEGF gene expression in the corpus luteum, the extraction of RNA, RT-PCR and electrophoresis will be realized. The statistical comparisons will be submitted to unpaired t test realized and the variance analysis for parametric variables, and Mann-Whitney and Kruskal-Wallis tests for variables that are not parametric, differences will be considered when p <0.05. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANTOS, FRANCISLAINE ANELIZE GARCIA; FREIRE, SAMUEL APARECIDO; VIEIRA, DEYVID PARREIRA; PAPA, PAULA DE CARVALHO; DE BARROS, GABRIELA FERNANDES; CASTILHO, CALIE; GUABERTO, LUCIANA MACHADO; ARANTES DE SOUZA, LILIAN FRANCISCO; LAPOSY, CECILIA BRAGA; BARILLI NOGUEIRA, ROSA MARIA; et al. White tea intake interferes with the expression of angiogenic factors in the corpora lutea of superovulated rats. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, v. 53, n. 7, p. 1666-1671, . (10/20583-2, 10/20100-1)

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