Advanced search
Start date

In situ evaluation of the dental material degradation by dental biofilm

Grant number: 10/12847-0
Support Opportunities:Regular Research Grants
Duration: April 01, 2011 - March 31, 2013
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Regina Maria Puppin Rontani
Grantee:Regina Maria Puppin Rontani
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


The dental biofilm can biodegrade the surfaces of restorative materials and to adapt to changes in the oral environment. However, few is known about the precise structure of the biofilm and how it interacts with the restorative material, being a challenge to control its growth by antimicrobial agents or other strategies. This study will evaluate, in situ, biofilm/restorative material interactions through quantitative and qualitative analyses. Ten discs of each material analyzed (IPS Empress 2 - control, Filtek Supreme; Vitremer; Ketak molar Easymix; Tytin) will be made in a metal matrix of 5.0mm x 2.0mm. Roughness and microhardness of each specimens will be initially evaluated. Ten volunteers will be selected to use palatal devices containing specimens of each experimental group. The experiment will consist of two phases: first, the volunteers will use the palatal devices for 48 hours and in the second phase, for 14 days. After the experimental periods, laser microscopy confocal analyses will be performed to visualize the architecture and viability of the biofilm. The images will be analyzed using COMSTAT software, which will allow the evaluation of bio-volume, roughness coefficient and biofilm surface/volume ratio. In addition, the restorative materials will be analysed after interaction with the biofilm, using scanning electron microscopy (MEV), energy dispersive spectroscopy (EDS), roughness and final hardness tests. All the data will be submitted to statistical analysis. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items

Please report errors in scientific publications list by writing to: