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Development of methodologies for photodynamic therapy applications on fungal infections


The aim of this research project is to develop methodologies to study the photodinamic inactivation of Candida albicans in induced fungal infections in mice. Although several in vitro studies have demonstrated positive antifungal activities of photodynamic therapy, the application of this treatment in vivo requires adjustments of the conditions established in vitro. To reach the purpose of the present project, a mice model of vaginal Candida infection will be developed, in which photosensitizer concentration for its adequate distribution in tissue, irradiation parameters for effective antifungal effect, photodynamic inactivation of mutant C. albicans with overexpressed characteristics of drug resistance and the association of photodynamic therapy to drugs that inhibit those characteristics will be evaluated. In addition, the alterations in virulence of C. albicans induced by oxidative stress, after sublethal photodynamic therapy, will be investigated through in vitro tests and in vivo pathogenicity assay. (AU)

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Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PRATES, RENATO A.; KATO, ILKA T.; RIBEIRO, MARTHA S.; TEGOS, GEORGE P.; HAMBLIN, MICHAEL R.. Influence of multidrug efflux systems on methylene blue-mediated photodynamic inactivation of Candida albicans. Journal of Antimicrobial Chemotherapy, v. 66, n. 7, p. 1525-1532, . (10/13313-9)
SUZUKI, LUIS CLAUDIO; KATO, ILKA TIEMY; PRATES, RENATO ARAUJO; SABINO, CAETANO PADIAL; YOSHIMURA, TANIA MATEUS; SILVA, TAMIRES OLIVEIRA; RIBEIRO, MARTHA SIMOES. Glucose modulates antimicrobial photodynamic inactivation of Candida albicans in biofilms. Photodiagnosis and Photodynamic Therapy, v. 17, p. 173-179, . (10/13313-9)
SABINO, C. P.; GARCEZ, A. S.; NUNEZ, S. C.; RIBEIRO, M. S.; HAMBLIN, M. R.. Real-time evaluation of two light delivery systems for photodynamic disinfection of Candida albicans biofilm in curved root canals. Lasers in Medical Science, v. 30, n. 6, p. 1657-1665, . (10/13313-9)
MACHADO-DE-SENA, R. M.; CORREA, L.; KATO, I. T.; PRATES, R. A.; SENNA, A. M.; SANTOS, C. C.; PICANCO, D. A.; RIBEIRO, M. S.. Photodynamic therapy has antifungal effect and reduces inflammatory signals in Candida albicans-induced murine vaginitis. Photodiagnosis and Photodynamic Therapy, v. 11, n. 3, p. 275-282, . (10/13313-9)
NUNEZ, SILVIA CRISTINA; GARCEZ, AGUINALDO SILVA; KATO, ILKA TIEMY; YOSHIMURA, TANIA MATEUS; GOMES, LAERCIO; BAPTISTA, MAURICIO SILVA; RIBEIRO, MARTHA SIMOES. Effects of ionic strength on the antimicrobial photodynamic efficiency of methylene blue. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 13, n. 3, p. 595-602, . (10/13313-9)
KATO, ILKA TIEMY; PRATES, RENATO ARAUJO; SABINO, CAETANO PADIAL; FUCHS, BETH BURGWYN; TEGOS, GEORGE P.; MYLONAKIS, ELEFTHERIOS; HAMBLIN, MICHAEL R.; RIBEIRO, MARTHA SIMOES. Antimicrobial Photodynamic Inactivation Inhibits Candida albicans Virulence Factors and Reduces In Vivo Pathogenicity. Antimicrobial Agents and Chemotherapy, v. 57, n. 1, p. 445-451, . (10/13313-9)
BAPTISTA, ALESSANDRA; SABINO, CAETANO P.; NUNEZ, SILVIA C.; MIYAKAWA, WALTER; MARTIN, AIRTON A.; RIBEIRO, MARTHA S.. Photodynamic damage predominates on different targets depending on cell growth phase of Candida albicans. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 177, p. 76-84, . (10/13313-9)
NUNEZ, SILVIA C.; YOSHIMURA, TANIA M.; RIBEIRO, MARTHA S.; JUNQUEIRA, HELENA C.; MACIEL, CLEITON; COUTINHO-NETO, MAURICIO D.; BAPTISTA, MAURICIO S.. Urea enhances the photodynamic efficiency of methylene blue. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 150, n. SI, p. 31-37, . (10/13313-9, 13/07937-8, 12/50680-5)
ALVARENGA, LETICIA HEINECK; PRATES, RENATO ARAUJO; YOSHIMURA, TANIA MATEUS; KATO, ILKA TIEMY; SUZUKI, LUIS CLAUDIO; RIBEIRO, MARTHA SIMOES; FERREIRA, LUIS RODOLFO; DOS SANTOS PEREIRA, SILVIO ANTONIO; MARTINEZ, ELIZABETH FERREIRA; SABA-CHUJFI, EDUARDO. Aggregatibacter actinomycetemcomitans biofilm can be inactivated by methylene blue-mediated photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 12, n. 1, p. 131-135, . (05/01756-5, 10/13313-9)

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