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HIV chronic infection in children and adolescents: innate and adaptive responses to inactivated HIV particles

Grant number: 10/16513-9
Support Opportunities:Regular Research Grants
Duration: January 01, 2011 - December 31, 2013
Field of knowledge:Health Sciences - Medicine - Maternal and Child Health
Principal Investigator:Maria Marluce dos Santos Vilela
Grantee:Maria Marluce dos Santos Vilela
Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated researchers: Emanuel Borges Vitor Anjos ; Marcos Tadeu Nolasco da Silva ; Sandra Cecília Botelho Costa ; Taís Nitsch Mazzola

Abstract

The objective of this study is to characterize in HIV-infected children and adolescents the response of dendritic cells (DC) and lymphocytes to inactivated HIV particles. The number and function of lymphocytes (NKT, CD4+, CD8+, Treg and Th17) and plasmacytoid and myeloid DC will be evaluated by flow cytometry. The thymic function will be assessed by the number of TREC copies in peripheral blood mononuclear cells (PBMC) by Real Time PCR. PBMC will be incubated with aldrithiol-2-inactivated HIV to analyze the lymphoproliferative response by flow cytometry and cytokine production by ELISA in the presence of HIV particles. Co-cultures with T lymphocytes and monocyte-derived dendritic cells (MDDC) pulsed with inactivated HIV will be performed, and their cytokine production and mRNA expression will be evaluated by ELISA and Real Time PCR, respectively. The variables will be compared in individuals with controlled and uncontrolled HIV viral load by the statistic software SPSS. (AU)

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