| Grant number: | 10/50015-6 |
| Support Opportunities: | Research Projects - Thematic Grants |
| Duration: | January 01, 2011 - December 31, 2014 |
| Field of knowledge: | Biological Sciences - Genetics - Animal Genetics |
| Principal Investigator: | Maria Luiza Silveira Mello |
| Grantee: | Maria Luiza Silveira Mello |
| Host Institution: | Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Pesquisadores principais: | Benedicto de Campos Vidal |
| Associated scholarship(s): | 14/23842-0 - DNA methylation in HeLa cells after treatment with valproic acid,
BP.MS 13/15339-3 - Diabetes mellitus type I effects on polyploidization, DNA methylation and gene expression on mouse hepatocytes, BP.IC 12/20444-8 - Hyperglycemic memory effects on epigenetic marks in HepG2 cells, BP.PD 12/03238-5 - Effects of valproic acid and trichostatin A on chromatin organization and its regulation by epigenetic factors in HepG2 cells grown under hyperglycemic conditions, BP.DR |
Abstract
In interphase cells chromatin results from the integration of the structure, organization and supraorganization of nucleoprotein complexes. Chromatin harbors DNA - associated activities like the control of gene expression, replication, recombination and repair, thus playing a significant role in the cell "fate". Although huge methodological and technological advancements in Cell Biology are available today, chromatin has not as yet its complex structural and dynamic organization characteristics entirely understood. The attainment of these goals has been difficult due to compartmentalization of chromatin components into specific territories and participation of epigenetic processes in their organization and physiology. The present Project has the objective of contributing for advancements in this realm. The points to be studied refer to structural and organizational changes in chromatin and its components with aging and type I-diabetes mellitus and with experimental interference in epigenetic markers assumed to be present in chromatin territories of specific cell types. The interference proposed is assumed to arise by action of drugs that affect histone and DNA modification. The methodology to be used involves molecular biology assays, specific antibodies, microspectrophotometry or video image analysis, fluorescence microscopy, and infrared spectroscopic analysis. Our goal is not only a better understanding of the chromatin structure and organization in the selected models, but also their participation in the chromatin response to different physiological processes. (AU)
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