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Chromatin structure and organization with aging and diabetes compared to induced changes in epigenetic markers


In interphase cells chromatin results from the integration of the structure, organization and supraorganization of nucleoprotein complexes. Chromatin harbors DNA - associated activities like the control of gene expression, replication, recombination and repair, thus playing a significant role in the cell "fate". Although huge methodological and technological advancements in Cell Biology are available today, chromatin has not as yet its complex structural and dynamic organization characteristics entirely understood. The attainment of these goals has been difficult due to compartmentalization of chromatin components into specific territories and participation of epigenetic processes in their organization and physiology. The present Project has the objective of contributing for advancements in this realm. The points to be studied refer to structural and organizational changes in chromatin and its components with aging and type I-diabetes mellitus and with experimental interference in epigenetic markers assumed to be present in chromatin territories of specific cell types. The interference proposed is assumed to arise by action of drugs that affect histone and DNA modification. The methodology to be used involves molecular biology assays, specific antibodies, microspectrophotometry or video image analysis, fluorescence microscopy, and infrared spectroscopic analysis. Our goal is not only a better understanding of the chromatin structure and organization in the selected models, but also their participation in the chromatin response to different physiological processes. (AU)

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Scientific publications (15)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVARENGA, ELENICE M.; RODRIGUES, VERA L. C. C.; MORAES, ALBERTO S.; NAVES, LUISA S.; MONDIN, MATEUS; FELISBINO, MARINA B.; MELLO, MARIA LUIZA S.. Histone epigenetic marks in heterochromatin and euchromatin of the Chagas' disease vector, Triatoma infestans. ACTA HISTOCHEMICA, v. 118, n. 4, p. 401-412, . (10/50015-6)
FELISBINO, MARINA BARRETO; DA COSTA, THIAGO ALVES; VICCARI GATTI, MARIA SILVIA; SILVEIRA MELLO, MARIA LUIZA. Differential Response of Human Hepatocyte Chromatin to HDAC Inhibitors as a Function of Microenvironmental Glucose Level. Journal of Cellular Physiology, v. 231, n. 10, p. 2257-2265, . (12/03238-5, 10/50015-6)
ALVARENGA, ELENICE M.; MONDIN, MATEUS; RODRIGUES, VERA L. C. C.; ANDRADE, LARISSA M.; VIDAL, BENEDICTO DE CAMPOS; MELLO, MARIA LUIZA S.. Contribution of AT-, GC-, and methylated cytidine-rich DNA to chromatin composition in Malpighian tubule cell nuclei of Panstrongylus megistus (Hemiptera, Reduviidae). ACTA HISTOCHEMICA, v. 114, n. 7, p. 665-672, . (10/50015-6)
GHIRALDINI, FLAVIA GERELLI; VITOLO CRISPIM, ANA CAROLINA; SILVEIRA MELLO, MARIA LUIZA. Effects of hyperglycemia and aging on nuclear sirtuins and DNA damage of mouse hepatocytes. MOLECULAR BIOLOGY OF THE CELL, v. 24, n. 15, p. 2467-2476, . (10/50015-6)
FELISBINO, MARINA BARRETO; VICCARI GATTI, MARIA SILVIA; MELLO, MARIA LUIZA S.. Changes in Chromatin Structure in NIH 3T3 Cells Induced by Valproic Acid and Trichostatin A. Journal of Cellular Biochemistry, v. 115, n. 11, p. 1937-1947, . (09/11763-0, 10/50015-6)
GHIRALDINI, FLAVIA G.; SILVEIRA, ANDRE B.; KLEINJAN, DIRK A.; GILBERT, NICK; MELLO, MARIA LUIZA S.. Genomic profiling of type-1 adult diabetic and aged normoglycemic mouse liver. BMC Endocrine Disorders, v. 14, . (10/50015-6)
RODRIGUES, HENRIQUE F.; SOUZA, TAFAREL A.; GHIRALDINI, FLAVIA G.; MELLO, MARIA LUIZA S.; MORAES, ALBERTO S.. Increased Age Is Associated With Epigenetic and Structural Changes in Chromatin From Neuronal Nuclei. Journal of Cellular Biochemistry, v. 115, n. 4, p. 659-665, . (10/50015-6)
ALVARENGAA, ELENICE M.; MONDIN, MATEUS; MARTINS, JAMES A.; RODRIGUES, VERA L. C. C.; VIDAL, BENEDICTO C.; RINCONES, JOHANA; CARAZZOLLE, MARCELO F.; ANDRADE, LARISSA MARA; MELLO, MARIA LUIZA S.. Spatial distribution of AT- and GC-rich DNA within interphase cell nuclei of Triatoma infestans Klug. Micron, v. 42, n. 6, p. 568-578, . (10/50015-6)
GHIRALDINI, FLAVIA G.; SILVA, ISABELA S.; MELLO, MARIA LUIZA S.. Polyploidy and chromatin remodeling in hepatocytes from insulin-dependent diabetic and normoglycemic aged mice. Cytometry Part A, v. 81A, n. 9, p. 755-764, . (10/50015-6)
FELISBINO, MARINA BARRETO; TAMASHIRO, WIRLA M. S. C.; MELLO, MARIA LUIZA S.. Chromatin Remodeling, Cell Proliferation and Cell Death in Valproic Acid-Treated HeLa Cells. PLoS One, v. 6, n. 12, . (09/11763-0, 10/50015-6)
FELISBINO, MARINA BARRETO; ZIEMANN, MARK; KHURANA, ISHANT; OKABE, JUN; AL-HASANI, KEITH; MAXWELL, SCOTT; HARIKRISHNAN, K. N.; MARTINS DE OLIVEIRA, CAMILA BORGES; MELLO, MARIA LUIZA S.; EL-OSTA, ASSAM. Valproic acid influences the expression of genes implicated with hyperglycaemia-induced complement and coagulation pathways. SCIENTIFIC REPORTS, v. 11, n. 1, . (14/10198-5, 10/50015-6, 15/10356-2)
PERES, LARA M. C.; FALCO, JOSE RICARDO P.; AGUIRRA, THAISA J.; MELLO, MARIA LUIZA S.. Feulgen-DNA content and chromatin organization in the spermathecal glands of Apis mellifera (Hymenoptera, Apoidea) queens with aging. Apidologie, v. 45, n. 5, p. 601-609, . (10/50015-6)
SILVA, ISABELA S.; GHIRALDINI, FLAVIA G.; VERONEZI, GIOVANA M. B.; MELLO, MARIA LUIZA S.. Polyploidy and nuclear phenotype characteristics of cardiomyocytes from diabetic adult and normoglycemic aged mice. ACTA HISTOCHEMICA, v. 120, n. 2, p. 84-94, . (10/50015-6)
ALVARENGA, ELENICE M.; IMPERADOR, CARLOS H. L.; BARDELLA, VANESSA B.; RODRIGUES, VERA L. C. C.; MONDIN, MATEUS; CABRAL-DE-MELLO, DIOGO C.; MORAES, ALBERTO S.; MELLO, MARIA LUIZA S.. Histone acetylation and methylation marks in chromatin of Panstrongylus megistus (Hemiptera, Reduviidae). ACTA HISTOCHEMICA, v. 120, n. 6, p. 572-577, . (10/50015-6, 15/10356-2)

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