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Studies of cellular signaling and induction mechanisms of cellulases formation by the fungus Trichoderma reesei (Hypocrea jecorina)

Abstract

The filamentous fungus Trichoderma reesei (Hypocrea jecorina) produces and secretes a large amount of cellulases and hemicellulases that can be used in degradation of biomass components with application in biofuel production. Transcription of the major components of cellulase complex is induced not only by cellulose but also by a variety of disaccharides including lactose, cellobiose, and sophorose and is antagonized by the glucose. However, neither the nature of inducer nor the cellular signaling pathways are totally known. The aim of this project is understand the mechanisms of cellulases formation by the fungus T. reesei as well the mechanism of repression and the cellular signaling pathways involved in these process. As approach, genomic and proteomic techniques will be used. cDNA libraries will be construct in different conditions cited above and sequenced using the Next Generation Sequencing-NGS and the expression of the genes differentially expressed will be validated by Real Time PCR (RT-qPCR). The secretome and identification of phosphorylated proteins under the conditions cited will be analyzed by Differential Gel Electrophoresis 2-D fluorescence (DIGE). With obtained data, a model of global gene expression will be constructed using bioinformatics tools what will let a better understanding of gene expression behavior of cellulolytic enzymes produced by T. reesei contributing for its application in biofuel industry. (AU)

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VEICULO: TITULO (DATA)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMPOS ANTONIETO, AMANDA CRISTINA; CASTRO, LILIAN DOS SANTOS; SILVA-ROCHA, RAFAEL; PERSINOTI, GABRIELA FELIX; SILVA, ROBERTO NASCIMENTO. Defining the genome-wide role of CRE1 during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis. Fungal Genetics and Biology, v. 73, p. 93-103, . (10/15683-8, 13/04125-2)
MELLO-DE-SOUSA, THIAGO M.; RASSINGER, ALICE; PUCHER, MARION E.; CASTRO, LILIAN DOS SANTOS; PERSINOTI, GABRIELA F.; SILVA-ROCHA, RAFAEL; POCAS-FONSECA, MARCIO J.; MACH, ROBERT L.; SILVA, ROBERTO NASCIMENTO; MACH-AIGNER, ASTRID R.. The impact of chromatin remodelling on cellulase expression in Trichoderma reesei. BMC Genomics, v. 16, . (10/15683-8)
CASTRO, LILIAN DOS SANTOS; DE PAULA, RENATO G.; ANTONIETO, AMANDA C. C.; PERSINOTI, GABRIELA F.; SILVA-ROCHA, RAFAEL; SILVA, ROBERTO N.. Understanding the Role of the Master Regulator XYR1 in Trichoderma reesei by Global Transcriptional Analysis. FRONTIERS IN MICROBIOLOGY, v. 7, . (10/15683-8, 14/23653-2)
CARDOSO LOPES, FABYANO ALVARES; STEINDORFF, ANDREI STECCA; GERALDINE, ALAERSON MAIA; BRANDAO, RENATA SILVA; MONTEIRO, VALDIRENE NEVES; LOBO JUNIOR, MURILLO; GUEDES COELHO, ALEXANDRE SIQUEIRA; ULHOA, CIRANO JOSE; SILVA, ROBERTO NASCIMENTO. Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum. FUNGAL BIOLOGY, v. 116, n. 7, p. 815-824, . (10/15683-8)
CASTRO, LILIAN DOS SANTOS; CAMPOS ANTONIETO, AMANDA CRISTINA; PEDERSOLI, WELLINGTON RAMOS; SILVA-ROCHA, RAFAEL; PERSINOTI, GABRIELA F.; SILVA, ROBERTO NASCIMENTO. Expression pattern of cellulolytic and xylanolytic genes regulated by transcriptional factors XYR1 and CRE1 are affected by carbon source in Trichoderrna reesei. GENE EXPRESSION PATTERNS, v. 14, n. 2, p. 88-95, . (10/15683-8)
CASTRO, LILIAN DOS SANTOS; PEDERSOLI, WELLINGTON RAMOS; ANTONIO, AMANDA CRISTINA CAMPOS; STEINDORFF, ANDREI STECCA; SILVA-ROCHA, RAFAEL; MARTINEZ-ROSSI, NILCE M.; ROSSI, ANTONIO; BROWN, NEIL ANDREW; GOLDMAN, GUSTAVO H.; FAA, VITOR M.; et al. Comparative metabolism of cellulose, sophorose and glucose in Trichoderma reesei using high-throughput genomic and proteomic analyses. BIOTECHNOLOGY FOR BIOFUELS, v. 7, . (10/15683-8)

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