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Gene-modified-tissue engineering of bone: overexpression and inhibition of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs and RECK) by osteoblasts from human mesenchymal stem cells seeded on collagen-chitosan

Grant number: 10/08918-9
Support type:Research Grants - Young Investigators Grants
Duration: February 01, 2011 - January 31, 2016
Field of knowledge:Health Sciences - Dentistry
Principal researcher:Katiúcia Batista da Silva Paiva
Grantee:Katiúcia Batista da Silva Paiva
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):15/18661-9 - Evaluation of bone quality of human dental pulp stem cells seeded on biomaterials with potential application in bone bioengineering, BP.IC
15/11076-3 - Bone bioengineering: TIMP-1 gene modulation effect on human dental pulp stem cells seeded on collagen / chitosan in in vivo osteogenesis model, BP.IC
15/08960-9 - Bone bioengineering: TIMP-2 gene modulating effect on human dental pulp stem cells seeded on collagen/chitosan on in vivo osteogenesis model, BP.IC
+ associated scholarships 15/08884-0 - Bone bioengineering: TIMP-3 gene modulating effect on human dental pulp stem cells seeded on collagen/chitosan on in vivo osteogenesis model, BP.IC
15/09777-3 - Development of hydrogels based on hyaluronic acid/collagen/chitosan for bone bioengineering, BP.IC
15/08875-1 - Bone bioengineering: TIMP-4 gene modulating effect on human dental pulp stem cells seeded on collagen/chitosan on in vivo osteogenesis model, BP.IC
14/01395-1 - Osteogenesis in vitro and in vivo from human dental pulp stem cells: role of matrix metalloproteinases and their inhibitors, BP.MS
13/09976-0 - Potencial cell transformation in mesenchymal stem cells derived from human dental pulp stem cells, BP.IC
11/04342-8 - Caveolin-1 gene overexpression and interference: consequences for cell differentiation from human dental stem cells, BP.MS
11/00204-0 - Gene-modified-tissue engineering of bone: Overexpression and Inhibition of Matrix Metalloproteinases (MMPs) and their inhibitors (TIMPs and RECK) in osteoblasts from human mesenchymal stem cells seeded on collagen-chitosan, BP.JP - associated scholarships

Abstract

The urban concentration and greater longevity have provided the increased number of chronic diseases and injuries arising from violence, especially bone loss. The classical therapy is the use of autologous grafts, but alternatives such as allograft, alloplastic or xenogenous are part of the therapeutic arsenal of the clinician. However, it is know that all have advantages and disadvantages, resulting in the absence of an ideal material to repair these lesions. The tissue engineering aims to use the cells of patients, combined with appropriate scaffolds, and stimulate them with growth factors / differentiation and extracellular matrix molecules (ECM) to produce new tissue in vitro. Our group has been studying potential biomaterials and growth factors for bone bioengineering, as well as the role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs and RECK) in the development and bone repair and biomaterial-ECM interaction in vivo. This project intends to verify if changing gene expression, either by overexpression or inhibition (interference), of MMPs (-2, -9 and -14), capable of cleavage all components of ECM, as well as of their inhibitors, the TIMPs (-1, -2, -3 and -4) and RECK, can modulate osteogenesis in vitro and in vivo by modifying the rate of degradation of a synthetic substrate potentially applicable to the tissue bioengineering: the composite collagen-chitosan. Further investigation of other genes related to osteogenesis, ECM components and cell adhesion that may be differentially expressed in the presence of biomaterial, and interventions in gene expression will also be performed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA PAIVA, KATIUCIA BATISTA; GRANJEIRO, JOSE MAURO. Bone tissue remodeling and development: Focus on matrix metalloproteinase functions. Archives of Biochemistry and Biophysics, v. 561, n. SI, p. 74-87, . (10/08918-9, 11/00204-0)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.