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Molecular mechanisms involved in the skeletal muscle alterations in rats with heart failure submitted to aerobic training

Grant number: 10/10583-5
Support Opportunities:Regular Research Grants
Duration: September 01, 2010 - August 31, 2012
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Maeli Dal Pai
Grantee:Maeli Dal Pai
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated researchers:Antonio Carlos Cicogna ; Katashi Okoshi ; Robson Francisco Carvalho


Heart failure (HF) is characterized by reduced exercise tolerance due to early occurrence of fatigue and dyspnea. These symptoms occur in part due to changes in phenotype and atrophy of skeletal muscle. Physical training is considered a practical proposed and accepted to minimize changes in muscle in HF. Thus, understanding the cellular and molecular mechanisms involved in skeletal muscle alterations of this pathology may be important to the development of therapeutic interventions and the targeting of specific training programs. This study will investigate the mediators of atrophy, hypertrophy pathways and myogenic regulatory factors (MRFs) gene and protein expression in skeletal muscle of rats with HF induced by aortic stenosis (AoS), submitted to aerobic training. Male Wistar rats (90 to 100g) will be used (n=48). After 18 weeks of AoS surgery, part of the control and AoS animals will be sacrificed to prove left ventricular hypertrophy. The remaining animals (control and AoS) will be randomly divided into trained or not. The animals will be submitted to aerobic training on a treadmill for 10 weeks (5 days / week). The training speed will be relative to lactate threshold, obtained in the progressive test. At the end of the experimental period, animals will be weighed, sacrificed and the soleus and plantaris muscles collected. For morphological and morphometric analysis of muscle fibers will be performed HE staining. The evaluation of gene and protein expression will be performed by real time PCR (qPCR) and Western blotting, respectively. The animals' blood will be collected, centrifuged and the serum will be used for analyzing levels of IGF-1, TNF-± and interleukin-6 by the ultra-sensitive ELISA method. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUZA, RODRIGO W. A.; FERNANDEZ, GEYSSON J.; CUNHA, JOAO P. Q.; PIEDADE, WARLEN P.; SOARES, LUANA C.; SOUZA, PAULA A. T.; DE CAMPOS, DIJON H. S.; OKOSHI, KATASHI; CICOGNA, ANTONIO C.; DAL-PAI-SILVA, MAELI; et al. Regulation of cardiac microRNAs induced by aerobic exercise training during heart failure. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, v. 309, n. 10, p. H1629-H1641, . (10/10583-5, 10/06281-3)
SOUZA, RODRIGO W. A.; PIEDADE, WARLEN P.; SOARES, LUANA C.; SOUZA, PAULA A. T.; AGUIAR, ANDREO F.; VECHETTI-JUNIOR, IVAN J.; CAMPOS, DIJON H. S.; FERNANDES, ANA A. H.; OKOSHI, KATASHI; CARVALHO, ROBSON F.; et al. Aerobic Exercise Training Prevents Heart Failure-Induced Skeletal Muscle Atrophy by Anti-Catabolic, but Not Anabolic Actions. PLoS One, v. 9, n. 10, . (10/10583-5)

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