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Influence of the cerebellar histaminergic system on emotional memory in mice

Grant number: 09/17496-3
Support type:Regular Research Grants
Duration: April 01, 2010 - March 31, 2012
Field of knowledge:Humanities - Psychology - Physiological Psychology
Principal researcher:Rosana Mattioli
Grantee:Rosana Mattioli
Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil


Experimental evidences indicate that the role of the cerebellum may be more complex than the simple regulation of motor responses. Recent studies have demonstrated significant involvement of the cerebellum in the consolidation of emotional memory. The aim of this study is to investigate the actions of the cerebellar histaminergic neural system in the consolidation of emotional memory in mice. It will be carried out through three experiments with microinjection of histaminergic substances in the cerebellar vermis of mice that will be submitted to the Elevated Plus-Maze (EPM) and to an inhibitory avoidance test: experiment 1 with microinjection of histamine (HA) at doses of 0.54 nmol , 1.36 nmol, 2.72 nmol and 4.07 nmol; Experiment 2 combined with microinjection of H1 antagonist (chlorpheniramine) at a dose of 10.23 nmol and HA, and experiment 3 combined with microinjection of H2 antagonist (ranitidine) at a dose of 11, 4nmol and HA. The dose of HA in experiments 2 and 3 will be defined by the results of experiment 1. There will be an implant of the cannula in the cerebellar vermis of male mice (Swiss Albino) weighing between 25 and 30g. After 3 days of recovery, the behavioral tests in the EPM and inhibitory avoidance test will be perform. The test on EPM will be performing on two consecutive days, Trial1 (T1) and Trial2 (T2). Immediately after T1 animals will receive the drug treatment. The test sessions will be recorded for later analysis of the conventional categories and ethological measures [number of entries in open arms (OAE), enclosed arms (EAE) and total number of entries (TE), time spent in open arms (OAT), enclosed arms (EAT) and the center of the maze (CT), and their respective percentages]. The memory in mice is evaluated by the decline in exploration of open arms in the Trial2 (T1/T2). The inhibitory avoidance test will also be on two consecutive days of testing, Day1 (D1) and Day2 (D2) and pharmacological treatment with microinjection will be held immediately after D1. The experiments will be recorded and subsequently, the latencies will be quantified by the researcher through a television monitor. The increase in latencies will be considered indicative of an increase in memory retention. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GIANLORENCO, A. C. L.; CANTO-DE-SOUZA, A.; MATTIOLI, R.. Intra-cerebellar microinjection of histamine enhances memory consolidation of inhibitory avoidance learning in mice via H2 receptors. Neuroscience Letters, v. 557, n. B, p. 159-164, . (09/15836-1, 09/17496-3)
A.C.L. GIANLORENÇO; K.R. SERAFIM; A. CANTO-DE-SOUZA; R. MATTIOLI. Effect of histamine H1 and H2 receptor antagonists, microinjected into cerebellar vermis, on emotional memory consolidation in mice. Brazilian Journal of Medical and Biological Research, v. 47, n. 2, p. 135-143, . (09/17496-3, 09/15836-1)
GIANLORENCO, A. C. L.; SERAFIM, K. R.; CANTO-DE-SOUZA, A.; MATTIOLI, R.. Emotional memory consolidation impairment induced by histamine is mediated by H-1 but not H-2 receptors. Brain Research Bulletin, v. 89, n. 5-6, p. 197-202, . (09/15836-1, 09/17496-3)
GIANLORENCO, A. C. L.; CANTO-DE-SOUZA, A.; MATTIOLI, R.. Microinjection of histamine into the cerebellar vermis impairs emotional memory consolidation in mice. Brain Research Bulletin, v. 86, n. 1-2, p. 134-138, . (09/15836-1, 09/17496-3)
GIANLORENCO, A. C. L.; RIBOLDI, A. M.; SILVA-MARQUES, B.; MATTIOLI, R.. Cerebellar vermis H-2 receptors mediate fear memory consolidation in mice. Neuroscience Letters, v. 587, p. 57-61, . (09/15836-1, 09/17496-3)

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