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Correlation between lipid peroxidation and inflammatory markers profile in diabetes mellitus type 2 patients with chronic periodontal disease

Grant number: 07/08362-8
Support type:Regular Research Grants
Duration: December 01, 2008 - May 31, 2011
Field of knowledge:Health Sciences - Dentistry - Periodontology
Cooperation agreement: CNPq - PPSUS
Principal researcher:Silvana Regina Perez Orrico
Grantee:Silvana Regina Perez Orrico
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The aim of this study is to evaluate the influence of lipid peroxidation on the systemic and local inflammatory markers profile in patients with type 2 Diabetes mellitus (DM) and chronic periodontitis. The sample will be composed of 90 patients subdivided into three groups: Group 1 (diabetes with good metabolic control), Group 2 (diabetes with poor metabolic control) and Group 3 (control/ systemically healthy). The metabolic control of the groups 1 and 2 will be evaluated by the glycated hemoglobin (HbA1c), being categorized as diabetes with poor metabolic control when HbA1c ³ 8.0%. All the subjects will go through a complete periodontal examination and physical and laboratory evaluation in order to verify fasting plasma glucose, lipid profile and levels of lipid peroxidation, that can be expressed by the levels of oxidized LDL and Malondialdehyde (MDA). Periodontal examination will consist of visible plaque index, gingival bleeding index, bleeding on probing, probing depth (PD) and clinical attachment levels (CAL). Samples of gingival crevicular fluid will be collected in 4 sites without periodontal disease (PD d 3mm, without loss of attachment and with no bleeding on probing) and 4 sites with periodontal disease (PD 5mm, CAL e 4mm, with bleeding on probing), in order to evaluate the quantity of metalloproteinase (MMP)-8, -9, -13, interleukin (IL)-1, -4, -5, -6, -8, -10, -12, -18, -32 and tumor necrosis factor-alpha (TNF- ±). In plasma it will be measured the quantity of IL-1², -2, -4, -5 -6, -10, -12, -32 e TNF-a. The groups will be compared as the concentration of the cytokines and lipid peroxidation levels (oxidized LDL and MDA) in gingival crevicular fluid and plasma. A statistical test will be applied to evaluate the correlation between the levels of lipid peroxidation and the concentration of each cytokine. This study may contribute for a better understanding of the conditions related to the greater severity of periodontal disease in patients with diabetes. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASTOS, ALLINY DE SOUZA; GRAVES, DANA T.; DE MELO LOUREIRO, ANA PAULA; ROSSA JUNIOR, CARLOS; TFAILE CORBI, SAMIA CRUZ; FRIZZERA, FAUSTO; SCAREL-CAMINAGA, RAQUEL MANTUANELI; CAMARA, NIELS OLSEN; ANDRIANKAJA, OELISOA M.; HIYANE, MEIRE I.; et al. Diabetes and increased lipid peroxidation are associated with systemic inflammation even in well-controlled patients. JOURNAL OF DIABETES AND ITS COMPLICATIONS, v. 30, n. 8, p. 1593-1599, . (07/08362-8)
NEPOMUCENO, RAFAEL; VILLELA, BARBARA SCORALICK; TFAILE CORBI, SAMIA CRUZ; BASTOS, ALLINY DE SOUZA; DOS SANTOS, RAQUEL ALVES; TAKAHASHI, CATARINA SATIE; PEREZ ORRICO, SILVANA REGINA; SCAREL-CAMINAGA, RAQUEL MANTUANELI. Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro-and Anti-Inflammatory Genes. Mediators of Inflammation, . (07/08362-8, 09/16233-9, 10/10882-2)
TFAILE CORBI, SAMIA CRUZ; BASTOS, ALLINY SOUZA; NEPOMUCENO, RAFAEL; CIRELLI, THAMIRIS; DOS SANTOS, RAQUEL ALVES; TAKAHASHI, CATARINA SATIE; ROCHA, CRISTIANE S.; PEREZ ORRICO, SILVANA REGINA; MAURER-MORELLI, CLAUDIA V.; SCAREL-CAMINAGA, RAQUEL MANTUANELI. Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus. JOURNAL OF DIABETES RESEARCH, . (07/08362-8, 09/16233-9, 10/10882-2)
NEPOMUCENO, RAFAEL; VALLERINI, BRUNA DE E.; DA SILVA, ROMERITO L.; CORBI, SARNIA C. T.; BASTOS, ALLINY DE S.; DOS SANTOS, RAQUEL A.; TAKAHASHI, CATARINA S.; ORRICO, SILVANA REGINA P.; SCAREL-CAMINAGA, RAQUEL M.. Systemic expression of genes related to inflammation and lipid metabolism in patients with dyslipidemia, type 2 diabetes mellitus and chronic periodontitis. DIABETES & METABOLIC SYNDROME-CLINICAL RESEARCH & REVIEWS, v. 13, n. 4, p. 2715-2722, . (07/08362-8, 09/16233-9, 14/19699-7, 10/10882-2)
BASTOS, ALLINY S.; GRAVES, DANA T.; DE MELO LOUREIRO, ANA PAULA; ROSSA JUNIOR, CARLOS; PARRA ABDALLA, DULCINEIA SAES; SANTO FAULIN, TANIZE DO ESPIRITO; CAMARA, NIELS OLSEN; ANDRIANKAJA, OELISOA M.; ORRICO, SILVANA R. P.. Lipid Peroxidation Is Associated with the Severity of Periodontal Disease and Local Inflammatory Markers in Patients with Type 2 Diabetes. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, v. 97, n. 8, p. E1353-E1362, . (07/08362-8)
CORBI, SAMIA C. T.; DE VASCONCELLOS, JAIRA F.; BASTOS, ALLINY S.; BUSSANELI, DIEGO GIROTTO; DA SILVA, BARBARA ROQUE; SANTOS, RAQUEL ALVES; TAKAHASHI, CATARINA S.; ROCHA, CRISTIANE DE S.; CARVALHO, BENILTON DE SA; MAURER-MORELLI, V, CLAUDIA; et al. Circulating lymphocytes and monocytes transcriptomic analysis of patients with type 2 diabetes mellitus, dyslipidemia and periodontitis. SCIENTIFIC REPORTS, v. 10, n. 1, . (07/08362-8, 14/16148-0, 10/10882-2, 09/16233-9)
BASTOS, ALLINY SOUZA; DE MELO LOUREIRO, ANA PAULA; DE OLIVEIRA, TIAGO FRANCO; TFAILE CORBI, SAMIA CRUZ; SCAREL CAMINAGA, RAQUEL MANTUANELI; ROSSA JUNIOR, CARLOS; ORRICO, SILVANA R. P.. Quantitation of malondialdehyde in gingival crevicular fluid by a high-performance liquid chromatography-based method. Analytical Biochemistry, v. 423, n. 1, p. 141-146, . (07/08362-8)

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