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Effects of orthodontic movement on the expression of inflammatory mediators in periodontal tissue of rats with induced periodontal disease or healthy periodontium

Grant number: 08/06328-0
Support Opportunities:Regular Research Grants
Duration: February 01, 2009 - January 31, 2011
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Joni Augusto Cirelli
Grantee:Joni Augusto Cirelli
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Orthodontic movement occurs in response to mechanical forces through periodontal tissues remodelling. Several chemical mediators are synthesized and released for the beginning and maintenance of tissue remodelling. Among these mediators, nitric oxide (NO) acts as modulator of osteoclasts and osteoblasts activities in bone turnover. Interleukin-1 (IL-1-²), interleukin-6 (IL-6) e tumor necrosis factor-± (TNF-±) stimulate bone resorption and are increased in orthodontic movement. These same signaling molecules are also increased and play an important role on tissue destruction in periodontal disease. The aim of this study is to assess in vivo the expression of IL-1ß, IL-6, TNF-±, NO synthase constitutive endothelial (ecNOS), and inducible NO synthase (iNOS) in dental tissues during orthodontic tooth movement in rats suffering from ligature-induced periodontal disease or in periodontally health animals. Cytokines expressions will be correlated to histomorphometric changes on alveolar bone and other periodontal tissues. Ninety-three rats will be divided into four groups: 1- negative control (sham operated), 2 - ligature-induced periodontal disease, 3 - orthodontic movement, 4 - ligature-induced periodontal disease, followed by orthodontic movement. Biopsies of gingival tissue around the first molars will be removed for protein and mRNA expression analysis, respectively by, Western blot and RT-PCR. Histologic tissue sections will be obtained for histomorphometric evaluation of alveolar bone loss, counting of inflammatory cells and immunohistochemistry analysis for chemical mediators localization. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BOAS NOGUEIRA, ANDRESSA VILAS; CHAVES DE SOUZA, JOAO ANTONIO; KIM, YEON JUNG; DE SOUSA-NETO, MANOEL DAMIAO; CIRELLI, CAROLINA CHAN; CIRELLI, JONI AUGUSTO. Orthodontic Force Increases Interleukin-1 beta and Tumor Necrosis Factor-alpha Expression and Alveolar Bone Loss in Periodontitis. Journal of Periodontology, v. 84, n. 9, p. 1319-1326, . (10/07771-4, 08/02506-0, 08/06328-0)

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