Effects of smoking in osteogesis on different titanium surfaces: an in vivo and in vitro study

Abstract

Biomaterials topography may influence different cell and tissues activities. Early publications allow that treatments provide different micro and nanotopography. Microtopographies may stimulate osteogenesis with a tridimensional environment that enhances cell-matrix interactions. However, there are local factors and systemic conditions able to interfere in formation and osseointegration maintenance. Smoke is one factor associated to implants failure. Animal's studies evaluated, with histological methods, the effect of smoke/nicotine in the bone/implant interface and show that the intermittent inhalation may result in less bone implant contact. The effect of surface modification over osseointegration, in the presence of local factors like smoke habit, is still a less understood question. Studies show less trabecular bone formation in smoking patients, and implant failure may be a late consequence after loading. Surface modifications may improve bone formation prevent or reducing this possible failure. Surfaces with nanotopography, microtopography and machined, will be tested in this study. In vivo histomorfometrics evaluations, in rats, and in vitro evaluations, using calvaria-derived osteogenic cultures, will be realized to understand the relation of different surfaces and the smoking habit/nicotine. (AU)