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Stress oxidative markers before and after periodontal treatment: smokers and non-smokers

Grant number: 08/05528-5
Support Opportunities:Regular Research Grants
Duration: June 01, 2009 - November 30, 2011
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Francisco Emílio Pustiglioni
Grantee:Francisco Emílio Pustiglioni
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Periodontal disease is the result of complex interactions between pathogenic bacteria and the host's immunoinflammatory responses. An important factor that can be involved in the progression of the periodontal disease is the production of Reactive Oxigen Species (ROS). The imbalance between oxidative stress induced by ROS and the concentrations (or activity) of the antioxidant may result in tissue damage. The ROS induce the activity of lipid peroxidation-Tbars, myeloperoxidase (MPO) and arginase, whose mechanisms reflect the severity of the periodontal disease and it may offer the basis for a patient specific diagnostic test for periodontitis and could have therapeutic significance. The aim of this study is to investigate quantitatively the levels of oxidative stress markers (Myeloperoxidase, Lipid Peroxidation and Arginase) in saliva and in gingival crevicular fluid (GCF) of smokers and not smokers patients with generalized chronic periodontitis, before and after periodontal treatment. It will be collected for 5 min, unstimulated whole saliva and GCF of 50 smokers patients and not smokers generalized chronic periodontitis patients before and after treatment and of 50 clinically health patients smokers and non smokers as controls. The clinical parameters of probing pocket depth, probing attachment level, plaque index and the gingival index will be evaluated. The activity of stress markers in saliva and GFC will be analyzed by spectrofotometria. Data will be analysed statistically by a comparative test that will be defined latter, assigning significance for two-tail p < 0.05. (AU)

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