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Adaptive physiological strategies in the Crustacea: the invasion of fresh water and the molecular and structural bases of osmoregulation

Abstract

The survival of aquatic organisms has resulted in part from the selection of regulatory mechanisms that maintain the osmotic and ionic equilibria of their internal milieu, thus protecting tissues and cells against the intervening gradient and oscillations in the external medium. The Crustacea have developed many adaptive osmotic and ionic patterns that underlie their ample distribution and notable diversity; thus, crustaceans are often used as comparative models to better comprehend osmoregulatory mechanisms that employ ion transporting proteins such as the Na+/K+- and V-ATPase which participate in volume regulation and ionic composition of the intra- and extracellular fluids. The palaemonid shrimps and brachyuran crabs exhibit numerous adaptive strategies clearly manifest in their life cycles, physiology, biochemistry and morphology that reflect the wide variety of environments occupied, and include species particularly useful to analyze adaptive osmoregulatory strategies. This investigation compares the levels of mRNA expression of the α and B subunits of the Na+/K+- and V-ATPase, the Na+/H+ exchanger and the Na+K+2Cl- symporter, and Na+/K+- and V-ATPase activity, in the gills of a series of marine, estuarine and freshwater palaemonid shrimps (Palaemon northropi, Macrobrachium acanthurus e M. brasiliense, respectively) and in the marine crab Callinectes danae as a function of the external medium salinity. Gill Na+/K+- and V-ATPase activity also will be localized by immunofluorescence microscopy in M. amazonicum and in C. danae in which the ultrastructure of the posterior gill ion-transporting cells will be characterized after adaptation to dilute salinity. The data generated will aid in clarifying the molecular mechanisms and structural rearrangements that underpin osmotic and ionic regulation in the decapod Crustacea, and should help elucidate the molecular phylogeny of íon-transporting proteins in the Palaemonidae as well as illustrate the diversity of osmoregulatory mechanisms present in different crustacean taxa that confront similar osmotic challenges. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FARIA, SAMUEL COELHO; PROVETE, DIOGO BORGES; THURMAN, CARL LEO; MCNAMARA, JOHN CAMPBELL. Phylogenetic patterns and the adaptive evolution of osmoregulation in fiddler crabs (Brachyura, Uca). PLoS One, v. 12, n. 2, . (09/50799-0, 07/04870-9, 16/13949-7, 11/08852-0)
FALEIROS, ROGERIO OLIVEIRA; FURRIEL, ROSA P. M.; MCNAMARA, JOHN CAMPBELL. Transcriptional, translational and systemic alterations during the time course of osmoregulatory acclimation in two palaemonid shrimps from distinct osmotic niches. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 212, p. 97-106, . (07/04870-9)
FARIA, SAMUEL COELHO DE; AUGUSTO, ALESSANDRA SILVA; MCNAMARA, JOHN CAMPBELL. Intra- and extracellular osmotic regulation in the hololimnetic Caridea and Anomura: a phylogenetic perspective on the conquest of fresh water by the decapod Crustacea. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL, v. 181, n. 2, p. 175-186, . (07/04870-9)
FALEIROS, ROGERIO OLIVEIRA; GOLDMAN, MARIA HELENA S.; FURRIEL, ROSA P. M.; MCNAMARA, JOHN CAMPBELL. Differential adjustment in gill Na+/K+- and V-ATPase activities and transporter mRNA expression during osmoregulatory acclimation in the cinnamon shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). Journal of Experimental Biology, v. 213, n. 22, p. 3894-3905, . (07/04870-9)
GARCON, DANIELA P.; LEONE, FRANCISCO A.; FALEIROS, ROGERIO O.; PINTO, MARCELO R.; MORAES, CINTYA M.; FABRI, LEONARDO M.; ANTUNES, CLAUDIA D.; MCNAMARA, JOHN CAMPBELL. Osmotic and ionic regulation, and kinetic characteristics of a posterior gill (Na+, K+)-ATPase from the blue crab Callinectes danae on acclimation to salinity challenge. Marine Biology, v. 168, n. 6, . (15/00131-3, 07/04870-9, 16/25336-0)
MELO FURRIEL, ROSA PRAZERES; SILVA FIRMINO, KELLY CRISTINA; MASUI, DOUGLAS CHODI; FALEIROS, ROGERIO OLIVEIRA; TORRES, ANTONIO HERNANDES; MCNAMARA, JOHN CAMPBELL. Structural and Biochemical Correlates of Na+, K+-ATPase Driven Ion Uptake Across the Posterior Gill Epithelium of the True Freshwater Crab, Dilocarcinus pagei (Brachyura, Trichodactylidae). JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY, v. 313A, n. 8, p. 508-523, . (07/04870-9)
SILVA FIRMINO, KELLY CRISTINA; FALEIROS, ROGERIO OLIVEIRA; MASUI, DOUGLAS CHODI; MCNAMARA, JOHN CAMPBELL; MELO FURRIEL, ROSA PRAZERES. Short- and long-term, salinity-induced modulation of V-ATPase activity in the posterior gills of the true freshwater crab, Dilocarcinus pagei (Brachyura, Trichodactylidae). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 160, n. 1, p. 24-31, . (07/04870-9)

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