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In vitro evaluation of the bioavailability of whey protein and its hydrolysates: enzimatic method complemented by cell culture

Grant number: 08/08252-0
Support Opportunities:Regular Research Grants
Duration: January 01, 2009 - June 30, 2011
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Rubens Monti
Grantee:Rubens Monti
Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Recently, milk constituents have been recognized as functional substances, suggesting that they might act in a direct and measurable way over humans' health. Between these constituents, special attention should be given to whey protein, a sub product obtained during cheese production which plays a major role in dairy industry, due to its production volume and nutritional composition. The present research project purpose is to verify, in vitro, the whey protein bioavailability, evaluating the hypothesis that these proteins, either integral or fractioned, could show different dialysability percentages, aiming to reveal the fraction that shows better bioavailability characteristics and allowing this fraction to be used in nutritional formulas, ensuring its functionality. For that there will be performed tests that simulate the digestion of the whole whey protein content, whey protein partially hydrolyzed and whey protein fully hydrolyzed, and that evaluate these compounds passage through membranes, determining the dialyzable proteins. In the next phase, CaCo-2 cellular cultures will be used to evaluate the intestinal absorption of these proteins. Both tests results will be quantitatively evaluated by high-performance liquid chromatography (HPLC), made with the samples obtained in each experiment. (AU)

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