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Analysis of the dynamics of alveolar wound healing filled with blood clot and bioactive glass (Perioglass) in non-diabetics, controlled diabetics and non controlled diabetics rats: a histologycal, histometrical and immunohistochemical study

Grant number: 07/00156-0
Support Opportunities:Regular Research Grants
Duration: April 01, 2007 - March 31, 2009
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Tetuo Okamoto
Grantee:Tetuo Okamoto
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The aim of this study is to evaluate the dynamics of the healing of dental sockets that were filled with blood clot or bioactive glass (Perio Glass) in non diabetics rats, in controlled diabetics rats and in non controlled diabetics rats, using the histologycal, histometrical and immunohistochemical as methodological approach. To perform this study, 136 male, Wistar rats, weighting between 250-280g will be divided in 3 groups: Group I (Control or Non Diabetic rats); Group II (non controlled diabetics rats) and Group III (controlled diabetics rats). The animals from Groups II and III will have the diabetics induced by Streptozotocin (STZ) administration. The STZ will be diluted in 0,1M Citrate Buffer, in penian vein, in concentration of 35mg/kg corporal Weight, while in Group I, the animals will receive in penian vein just the injection of Citrate Buffer. In the animals of Group III, insuline will be daily administrated in subcutaneous place. The glycemic taxes and corporal weight will be measured at each 4 days, until the end of experiment. At 12º. Day after the administration of STZ or Citrate Buffer, the animals will have the right upper incisive extracted. Half of the animals will have the dental socket filled with blood clot and sutured with polyglactin 910 thread; the other half of the animals will have the dental socket filled with Perioglass® and will be sutured with the same thread. The experimental periods will be at 7, 14 and 28 days after the surgery. The histologycal analysis will be qualitative, and the histometrical will be quantitative using the Image Lab Software (Duracom 3). The immunohistochemiscal reactions will be performed using primary antibodies to evidence Osteoprotegerin, RANK, RANKL and Osteocalcin, by immunoperoxidase as detection method and under optical microscopycal analysis. The fluorochromes used to evaluate the dynamic of calcium deposition (mineralization) will be alizarin red, calcein and oxytetracicline. The analysis will be performed with an epifluorescence mycroscope, with specific filters with the lenght wave for each fluorochrome. (AU)

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