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Comparison of fluorescent antibody test and RT-PCR in brain of dogs with canine distemper and genetic characterization of virus strains detected

Grant number: 06/04924-9
Support Opportunities:Regular Research Grants
Duration: February 01, 2007 - January 31, 2010
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Valor Concedido/Desembolsado (R$): 54,611.55 / 54,611.55
Principal Investigator:Jane Megid
Grantee:Jane Megid
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Canine distemper is an infectious disease characterized by several clinical symptoms that obligate differencial diagnosis. Fluorescent antibody test has as problem the central nervous system irregular distribution of canine distemper virus. In addition, several virus strain have been described associated to more sensible diagnostic techniques as well as PCR. The aim of this work evaluate the PCR fluoresce comparetively to FAT in post morten diagnosis as well as to sequence the virus strain in relation to vaccinal strains normally used. (AU)

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