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Molecular cell biology of oligopeptidases

Grant number: 04/04933-2
Support Opportunities:Research Projects - Thematic Grants
Duration: October 01, 2004 - September 30, 2009
Field of knowledge:Biological Sciences - Morphology - Cytology and Cell Biology
Principal Investigator:Emer Suavinho Ferro
Grantee:Emer Suavinho Ferro
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Pesquisadores principais:
Stephen Hyslop

Abstract

Endo-oligopeptidases is a denomination coined by Camargo and cols. (OLIVEIRA, et al., 1976) to describe the substrate specificity of two enzymes named endo-oligopeptidases A and B, which only cleaves short peptides (from 5-17 amino acids). At this time we intend to keep on investigating the cell biology and function of endo-oligopeptidases EP24.15 (EC 3.4.24.15) and EP24.16 (EC 3.4.24.16), renewing the financial support received from FAPESP (grants 96/05904-8 and 99/01983-9).The primarily intracellular location (e.g., cytosolic, nuclear, mitochondrial) of peptidases such as EP24.15 (EC 3.4.24.15; Fontenele-Neto et al. 2001) and EP24.16 (EC 3.4.24.16; Checler et al. 1995; Kato et aI. 1997), suggests additional functions besides extracellular neuropeptide/hormone metabolism/processing. In collaboration with the laboratories of Professors Antonio C.M. Camargo (Instituto Butantan) and Célio Silva (FMRP, U8P), we have shown that oligopeptidases such as EP24.15 play an important intracellular role in degrading peptides released by the 268 proteasome; others further confirmed these original data (FERRO et al., submitted). Thus, the aim of the present Projeto Temático is to investigate the molecular cell biology of endo-oligopeptidases EP24.15 and EP24.16. (AU)

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Scientific publications (21)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GEWEHR, MAYARA C. F.; TEIXEIRA, ALEXANDRE A. S.; SANTOS, BRUNA A. C.; BIONDO, LUANA A.; GOZZO, FABIO C.; CORDIBELLO, AMANDA M.; EICHLER, ROSANGELA A. S.; RECKZIEGEL, PATRICIA; DA SILVA, RENEE N. O.; DOS SANTOS, NILTON B.; et al. The Relevance of Thimet Oligopeptidase in the Regulation of Energy Metabolism and Diet-Induced Obesity. BIOMOLECULES, v. 10, n. 2, . (14/17264-3, 16/04000-3, 04/04933-2, 15/20657-0)
DOS SANTOS, NILTON B.; FRANCO, ROSEANE D.; CAMARINI, ROSANA; MUNHOZ, CAROLINA D.; EICHLER, ROSANGELA A. S.; GEWEHR, MAYARA C. F.; RECKZIEGEL, PATRICIA; LLANOS, RICARDO P.; DALE, CAMILA S.; DA SILVA, VICTORIA R. O.; et al. Thimet Oligopeptidase (EC 3.4.24.15) Key Functions Suggested by Knockout Mice Phenotype Characterization. BIOMOLECULES, v. 9, n. 8, . (16/04000-3, 15/07273-8, 04/04933-2, 15/20657-0)
CUNHA, FERNANDA M.; BERTI, DENISE A.; FERREIRA, ZULMA S.; KLITZKE, CLECIO F.; MARKUS, REGINA P.; FERRO, EMER S.. Intracellular peptides as natural regulators of cell signaling. Journal of Biological Chemistry, v. 283, n. 36, p. 24448-24459, . (04/04933-2)
DEMASI, MARILENE; PIASSA FILHO, GILBERTO M.; CASTRO, LEANDRO M.; FERREIRA, JULIANA C.; RIOLI, VANESSA; FERRO, EMER S.. Oligomerization of the cysteinyl-rich oligopeptidase EP24.15 is triggered by S-glutathionylation. Free Radical Biology and Medicine, v. 44, n. 6, p. 1180-1190, . (04/04933-2, 06/06969-0)
CARRENO, FLÁVIA R.; GONI, CAMILA N.; CASTRO, LEANDRO M.; FERRO, EMER S.. 14-3-3 epsilon modulates the stimulated secretion of endopeptidase 24.15. Journal of Neurochemistry, v. 93, n. 1, p. 10-25, . (04/04933-2)
BEREZNIUK, IRYNA; SIRONI, JUAN; CALLAWAY, MYRASOL B.; CASTRO, LEANDRO M.; HIRATA, IZAURA Y.; FERRO, EMER S.; FRICKER, LLOYD D.. CCP1/Nna1 functions in protein turnover in mouse brain: Implications for cell death in Purkinje cell degeneration mice. FASEB JOURNAL, v. 24, n. 6, p. 1813-1823, . (04/04933-2)
GELMAN, JULIA S.; SIRONI, JUAN; CASTRO, LEANDRO M.; FERRO, EMER S.; FRICKER, LLOYD D.. Hemopressins and other hemoglobin-derived peptides in mouse brain: comparison between brain, blood, and heart peptidome and regulation in Cpefat/fat mice. Journal of Neurochemistry, v. 113, n. 4, p. 871-880, . (04/04933-2)
CASTRO, LEANDRO M.; BERTI, DENISE A.; RUSSO, LILIAN C.; COELHO, VERONICA; GOZZO, FABIO C.; OLIVEIRA, VITOR; FERRO, EMER S.. Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis. AAPS JOURNAL, v. 12, n. 4, p. 608-616, . (10/00828-0, 04/04933-2)
RUSSO, LILIAN C.; GONI, CAMILA N.; CASTRO, LEANDRO M.; ASEGA, AMANDA F.; CAMARGO, ANTONIO C. M.; TRUJILLO, CLEBER A.; ULRICH, HENNING; GLUCKSMAN, MARC J.; SCAVONE, CRISTOFORO; FERRO, EMER S.. Interaction with calmodulin is important for the secretion of thimet oligopeptidase following stimulation. FEBS Journal, v. 276, n. 16, p. 4358-4371, . (04/04933-2)
BERTI, DENISE A.; MORANO, CAIN; RUSSO, LILIAN C.; CASTRO, LEANDRO M.; CUNHA, FERNANDA M.; ZHANG, XIN; SIRONI, JUAN; KLITZKE, CLECIO F.; FERRO, EMER S.; FRICKER, LLOYD D.. Analysis of Intracellular Substrates and Products of Thimet Oligopeptidase in Human Embryonic Kidney 293 Cells. Journal of Biological Chemistry, v. 284, n. 21, p. 14105-14116, . (04/04933-2)
CASTRO, LEANDRO M.; CAVALCANTI, DIOGO M. L. P.; ARAUJO, CHRISTIANE B.; RIOLI, VANESSA; ICIMOTO, MARCELO Y.; GOZZO, FABIO C.; JULIANO, MARIA; JULIANO, LUIZ; OLIVEIRA, VITOR; FERRO, EMER S.. Peptidomic analysis of the neurolysin-knockout mouse brain. JOURNAL OF PROTEOMICS, v. 111, p. 11-pg., . (04/04933-2)
DE ARAUJO, CHRISTIANE B.; HEIMANN, ANDREA S.; REMER, RICARDO A.; RUSSO, LILIAN C.; COLQUHOUN, ALISON; FORTI, FABIO L.; FERRO, EMER S.. Intracellular Peptides in Cell Biology and Pharmacology. BIOMOLECULES, v. 9, n. 4, . (16/04000-3, 04/04933-2)
WARDMAN, JONATHAN H.; ZHANG, XIN; GAGNON, SANDRA; CASTRO, LEANDRO M.; ZHU, XIAORONG; STEINER, DONALD F.; DAY, ROBERT; FRICKER, LLOYD D.. Analysis of peptides in prohormone convertase 1/3 null mouse brain using quantitative peptidomics. Journal of Neurochemistry, v. 114, n. 1, p. 215-225, . (04/04933-2)
GOMES, IVONE; DALE, CAMILA S.; CASTEN, KIMBIE; GEIGNER, MIRIAM A.; GOZZO, FABIO C.; FERRO, EMER S.; HEIMANN, ANDREA S.; DEVI, LAKSHMI A.. Hemoglobin-derived Peptides as Novel Type of Bioactive Signaling Molecules. AAPS JOURNAL, v. 12, n. 4, p. 658-669, . (04/14258-0, 04/04933-2)
GOMES, IVONE; GRUSHKO, JULIA S.; GOLEBIEWSKA, URSZULA; HOOGENDOORN, SASCHA; GUPTA, ACHLA; HEIMANN, ANDREA S.; FERRO, EMER S.; SCARLATA, SUZANNE; FRICKER, LLOYD D.; DEVI, LAKSHMI A.. Novel endogenous peptide agonists of cannabinoid receptors. FASEB JOURNAL, v. 23, n. 9, p. 3020-3029, . (04/14258-0, 04/04933-2)
OLIVEIRA‚ V.; GARRIDO‚ P.A.G.; RODRIGUES‚ C.C.; COLQUHOUN‚ A.; CASTRO‚ L.M.; ALMEIDA‚ P.C.; SHIDA‚ C.S.; JULIANO‚ M.A.; JULIANO‚ L.; CAMARGO‚ A.; et al. Calcium modulates endopeptidase 24.15 (EC 3.4. 24.15) membrane association‚ secondary structure and substrate specificity. FEBS Journal, v. 272, n. 12, p. 2978-2992, . (04/04933-2)
GUPTA, ACHLA; DÉCAILLOT, FABIEN M.; GOMES, IVONE; TKALYCH, OLEG; HEIMANN, ANDREA S.; FERRO, EMER S.; DEVI, LAKSHMI A.. Conformation state-sensitive antibodies to G-protein-coupled receptors. Journal of Biological Chemistry, v. 282, n. 8, p. 5116-5124, . (04/14258-0, 04/04933-2)
MACHADO, M. F. M.; CUNHA, F. M.; BERTI, D. A.; HEIMANN, A. S.; KLITZKE, C. F.; RIOLI, V.; OLIVEIRA, V.; FERRO, E. S.. Substrate phosphorylation affects degradation and interaction to endopeptidase 24.15, neurolysin, and angiotensin-converting enzyme. Biochemical and Biophysical Research Communications, v. 339, n. 2, p. 518-523, . (04/04933-2)
DALE, CAMILA SQUARZONI; PAGANO, ROSANA DE LIMA; RIOLI, VANESSA; HYSLOP, STEPHEN; GIORGI, RENATA; FERRO, EMER SUAVINHO. Antinociceptive action of hemopressin in experimental hyperalgesia. Peptides, v. 26, n. 3, p. 431-436, . (04/04933-2)
CAVALCANTI, DIOGO M. L. P.; CASTRO, LEANDRO M.; ROSA NETO, JOSE C.; SEELAENDER, MARILIA; NEVES, RODRIGO X.; OLIVEIRA, VITOR; FORTI, FABIO L.; IWAI, LEO K.; GOZZO, FABIO C.; TODIRAS, MIHAIL; et al. Neurolysin Knockout Mice Generation and Initial Phenotype Characterization. Journal of Biological Chemistry, v. 289, n. 22, p. 15426-15440, . (04/04933-2)
CASTRO, LEANDRO M.; CAVALCANTI, DIOGO M. L. P.; ARAUJO, CHRISTIANE B.; RIOLI, VANESSA; ICIMOTO, MARCELO Y.; GOZZO, FABIO C.; JULIANO, MARIA; JULIANO, LUIZ; OLIVEIRA, VITOR; FERRO, EMER S.. Peptidomic analysis of the neurolysin-knockout mouse brain. JOURNAL OF PROTEOMICS, v. 111, n. SI, p. 238-248, . (04/04933-2)

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