Conditional depletion of AMPA receptor subunits (GluA1 and GluA4) in spinal cord astrocytes and the impact after proximal axotomy

Abstract

This proposal seeks to deepen our understanding of the complex interplay between glial cells and neuronal function following traumatic injury. Recent research has illuminated the morpho-functional changes occurring in spinal motoneurons following trauma, revealing molecular and metabolic alterations influenced by interactions with the microenvironment. Yet, gaps persist in understanding the role of glial cells, particularly astrocytes, in modulating these responses. Glutamatergic signaling, mediated by AMPA-type ionotropic glutamate receptors, emerges as a key player in excitotoxicity and neuronal survival post-injury. Building upon ongoing investigations supported by FAPESP, this proposal outlines collaborative exchange activities between the University of Campinas (UNICAMP) and the Universität des Saarlandes (University of Saarland), Campus Homburg (USAAR) aimed at elucidating these mechanisms. Our efforts include workshops, courses, and scientific meetings to foster knowledge exchange and collaboration. Activities planned in the partner institution include confirming recombination efficiency in spinal astrocytes by the use of advanced techniques such as MACS sorting of spinal astrocytes, and qRT-PCR. Through these collaborative efforts, we aim to bridge existing knowledge gaps and pave the way for targeted interventions to improve outcomes following proximal motoneuron axotomy. This initiative holds promise not only for advancing scientific understanding but also for fostering sustained collaboration between research institutions in Brazil and beyond. (AU)