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Muscle regeneration: association of telocytes and satellite cells in the plasticity of the myotendinous junction

Grant number: 22/04272-4
Support Opportunities:Regular Research Grants
Duration: March 01, 2023 - February 28, 2025
Field of knowledge:Biological Sciences - Morphology - Cytology and Cell Biology
Principal Investigator:Adriano Polican Ciena
Grantee:Adriano Polican Ciena
Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated researchers:II-Sei Watanabe

Abstract

The interface between muscle and tendon tissues is denominated the myotendinous junction (JMT), a highly complex and specialized region that presents wide and differentiated plasticity in the face of various stimuli. The present study aims to investigate the association of telocytes with satellite cells in the JMT region in an experimental model in different post-muscle injury phases, and thus reveal their association and possible communication between these cells during the regenerative process. Will be used 100 adult Wistar rats divided into 2 experimental groups, the Control Group and Muscle Injury Group (LM). The LM group will be subdivided into 4 post-injury subgroups (LM0h, LM24h, LM48h, LM7d). To reveal the structural alterations of the JMT and the muscle belly, light microscopy analysis will be performed. Qualitative molecular investigations will occur through the immunofluorescence technique of muscle fiber types (Myosin Skeletal Fast and Troponin I Skeletal Slow), telocytes (PDGFR-± and CD34), and satellite cells (PAX7, MyoD, and myogenin) in JMT. In addition, the primary cell culture of telocytes and associated satellite cells will be performed for the isolation of their extracellular vesicles. While, quantitative analyzes will be established by obtaining the protein expression of PDGFR-±, Myf5, Murf-1, Pax7, Atrogin-1, and GAPDH, and by analyzing gene expression using the real-time PCR technique, for PDGFR-±, CD34, Murf1, Atrogin-1, Pax7, MyoD, myogenin, IL-6, and IGF. (AU)

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